discontinued

DM002 Actin (pan alpha / gamma 2) antibody

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1 ml / €470.00

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Mouse anti Human, Rabbit, Rat Actin (pan alpha / gamma 2) HHF35

Product Description for Actin (pan alpha / gamma 2)

Mouse anti Human, Rabbit, Rat Actin (pan alpha / gamma 2) HHF35.
Presentation: Supernatant
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Actin (pan alpha / gamma 2)

Product Category Primary Antibodies
Quantity 1 ml
Presentation Supernatant
Reactivity Hu, Rb, Rt
Applications P, WB
Clonality Monoclonal
Clone HHF35
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
SDS extracted protein from human myocardium
Application Immunohistochemistry on formalin fixed paraffin embedded sections: use a dilution of 1:25-1:75 in an ABC method (30 minutes at room temperature).
Recommended positive control: Muscle or sarcoma.
Western blotting.
General Readings Gowm et al. Am J Pathol 125: 191, 1986.
Tsukada et al. Am J Pathol 126: 51, 1987.
Porter et al. Mod Pathol 4: 46, 1991.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Liquid tissue culture supernatant containing sodium azide as preservative
Supernatant
Specificity
Specificity:
The antibody is specific to actin from tissue extracts of uterus, ileum, aorta, diaphragm, heart, and smooth muscle cells. It recognizes the alpha actin from skeletal, cardiac, and smooth muscle and the gamma actin from smooth muscle sources. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Cellular Localization: Cytoplasmic.
In Western blot, clone HHF35 reacts with 42 kD protein extract from skeletal muscle but not from epithelial cell line.
Species:
Human, rabbit, rat.
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