BM5093B Adeno-Associated Virus 1 / AAV1 (intact particle) antibody

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0.75 ml / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Adeno-Associated Virus 1 / AAV1 ADK1a

BM5093B

Product Description for Adeno-Associated Virus 1 / AAV1

Mouse anti Adeno-Associated Virus 1 / AAV1 ADK1a.
Properties: (intact particle)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay.

Properties for Adeno-Associated Virus 1 / AAV1

Product Category Primary Antibodies
Quantity 0.75 ml
Synonyms AAV-1
Presentation Biotin
Applications C, E
Clonality Monoclonal
Clone ADK1a
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Adeno-associated virus type 1 (AAV-1) capsid proteins and virus particles
Property intact particle
Isotype control AM03096PU-N
Application ELISA.
Immunohistochemistry.
Not Suitable for Immunoblotting.
Dilution Buffer: PBS or Tris-buffered saline. for ELISA use PBS with 0.05% Tween-20
Working Dilutions: After reconstitution with 750 μl distilled water, dilute 1/20 for ELISA (dilute immediately before use).
Incubation Time: 1 h at 37°C For ELISA and overnight at 2-8°C for Immunohistochemistry or Immunofluorescence microscopy.
General Readings
  1. Kuck D, Kern A, Kleinschmidt JA. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J Virol Methods. 2007 Mar;140(1-2):17-24. Epub 2006 Nov 28. PubMed PMID: 17126418.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Biotin
Purification:
Affinity Chromatography
Preservatives:
0.09% Sodium Azide
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore with 750 μl distilled water to a concentration of 2.5 μg/ml.
Species Reactivity
Species reactivity (tested):
AAV-1, found in Human and Monkey.
Specificity
Specificity:
For characterization of different stages of infection and Specificity very useful for the analysis of the AAV assembly process.
ADK1a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present
in denatured capsid proteins and native but unassembled capsid proteins. 
In immunofluorescence microscopy and dot blot analysis cross-reactivity with AAV-6 was observed. No crossreaction with AAV type 2, 3, 4, 5.

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