DM3002 Adenovirus Fiber (Monomer and Trimer) antibody

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Mouse anti Adenovirus Fiber (Monomer and Trimer) 4D2


Product Description for Adenovirus Fiber (Monomer and Trimer)

Mouse anti Adenovirus Fiber (Monomer and Trimer) 4D2.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Adenovirus Fiber (Monomer and Trimer)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3233
Presentation Purified
Applications ICC/IF, WB
Clonality Monoclonal
Clone 4D2
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

UV-irradiated Ad2 virus
Isotype control AM03096PU-N
Application Immunofluorescence (1). Western Blotting (1). Recommended positive control: Adenovirus infected cells and tissues. Not suitable for Inhibition of Ad2 attachment (2). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Hong JS, Engler JA. The amino terminus of the adenovirus fiber protein encodes the nuclear localization signal. Virology. 1991 Dec;185(2):758-67. PubMed PMID: 1962447.
  2. Hong, J.S., En
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
This Ab recognizes both monomers (62kDa) and trimers (180-200kDa), as judged by reactivity with both boiled (monomers only) and unboiled (both monomers and trimers) fiber on Western blots and by indirect immuno-fluorescence studies of cells infected with a temperaturesensitive Ad5 fiber mutant (H5ts142) which does not form trimers at the nonpermissive temp. at both 32 C and 39 C(1).
Adenovirus capsid proteins (62 kD and 180-200 kD) are synthesized in the cytoplasm and transported to the nucleus for assembly into the virus particles. The three major capsid proteins (hexon, penton base, and fiber) are synthesized late in infection. Reacts with Adenovirus infected cells and tissues. Cellular Localization: nuclear.

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