AM00315PU-N Adenovirus Type 2 + 5 (E1A) antibody

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Mouse anti Adenovirus Type 2 M58+M73

Product Description for Adenovirus Type 2

Mouse anti Adenovirus Type 2 M58+M73.
Properties: + 5 (E1A)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Adenovirus Type 2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Adenovirus antibody
Presentation Purified
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone M58+M73
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Property + 5, E1A
Isotype control AM03096PU-N
Application Immunofluorescence.
Immunoprecipitation (Native verified): Use Protein A; Ab 2 µg/mg protein lysate.
Western Blotting: Ab at 1µg/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2µg/ml for 30 minute at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Recommended positive control: 293 Cells or Adenovirus infected tissues.
Background Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.

The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46 kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
Concentration 0.2 mg/ml
General Readings
  1. Harlow E, et al. (1986)Mol Cell Biol, 6:1579-1589
  2. Harlow E, et al. (1985) J Virol, 3:533-546.
  3. Whyte P, et al. (1988) Nature, 334:124-129
  4. Klein g, et al. (1987) Science 238:1539-1544
Storage Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
Liquid ascites
This Adenovirus antibody detects Adenovirus Type 2 + 5 E1A.
Cellular Localization: Nuclear.

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