DM3005 Adenovirus Type 5 E1A antibody

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Mouse anti Adenovirus Type 5 E1A M58


Product Description for Adenovirus Type 5 E1A

Mouse anti Adenovirus Type 5 E1A M58.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Adenovirus Type 5 E1A

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms AB3165
Presentation Purified
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone M58
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Isotype control AM03096PU-N
Application Immunofluorescence: 0.2-0.4µg/ml.
Immunoprecipitation: Native verified; Use Protein A; Ab at 2µg/mg protein lysate.
Western Blotting: (0.5-1.0µg/ml for 2 hrs at RT.
Immunohistochemistry on frozen and paraffin embedded sections: Use Ab at 2-4µg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended positive control: 293 Cells or Adenovirus infected cells and tissues.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Harlow E, et al. (1986)Mol Cell Biol, 6:1579-1589.
  2. Harlow E, et al. (1985) J Virol, 3:533-546.
  3. Whyte P, et al. 91988) Nature, 334:124-129.
  4. Klein g, et al. (1987) Scien
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Reacts with 293 cells or adenovirus infected cells and tissues. The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46 kD) with multifuctional capabilities and form a specific complex. Cellular Localization: nuclear.

Mol. Wt. of Antigen: 35-46 kD

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