discontinued

NB120-12358 Agrin antibody

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50 µg / €380.00

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Mouse anti Mouse, Rat Agrin Agr 33

Product Description for Agrin

Mouse anti Mouse, Rat Agrin Agr 33.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Agrin

Product Category Primary Antibodies
Quantity 50 µg
Presentation Purified
Reactivity Ms, Rt
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone Agr 33
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6PCM6
Immunogen:
Recombinant rat agrin, C-terminal construct.
GeneID:
11603
Application Immunocytochemistry, Western Blot, ICC: Use at a concentration of 2 ug/ml. WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 208.6 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., Immunocytochemistry, Western Blot
Background Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.
Concentration 1 mg/ml
General Readings General / background references:Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). PubMed PMID: 11349136
Hoch W Formation of the neuromuscular junction. Agrin and its unusual re
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
0.09% Sodium Azide
Purity:
protein G purified
State:
Purified
Specificity
Specificity:
Epitope has been mapped near the 2nd laminin domain homology region and causes a marked reduction in the number of nicotinic acetylcholine receptor (nAChR) clusters. Insertion of an 8aa peptide at splicing site Z increases the clustering activity, detects all isoforms of agrin.
Species:
Mouse, Rat
Gene ID 11603

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