TA319196 AHA1 / AHSA1 antibody

Rabbit polyclonal anti-AHA1 antibody

See related secondary antibodies

Search for all "AHA1 / AHSA1"

0.1 mg / €503.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Chimpanzee, Human AHA1 / AHSA1

Product Description for AHA1 / AHSA1

Rabbit anti Chimpanzee, Human AHA1 / AHSA1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for AHA1 / AHSA1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Activator of 90 kDa heat shock protein ATPase homolog 1, C14orf3, HSPC322
Presentation Aff - Purified
Reactivity Chimp, Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95433
Immunogen:
Synthetic peptide corresponding to an internal region of human AHA1 protein
GeneID:
10598
Application ELISA: 1/35,000 - 1/185,000.
Western Blot: 1/500 - 1/3,000.
Background Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist clientprotein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Concentration 1.08 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Panaretou B, Siligardi G, Meyer P, Maloney A, Sullivan JK, Singh S, et al. Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1. Mol Cell. 2002 Dec;10(6):1307-18. PubMed PMID: 12504007.
  2. Meyer P, Prodromou C, Liao C, Hu B, Roe SM, Vaughan CK, et al. Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery. EMBO J. 2004 Mar 24;23(6):1402-10. PubMed PMID: 15039704. (Free PMC Article available)
  3. Wang X, Venable J, LaPointe P, Hutt DM, Koulov AV, Coppinger J, et al. Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis. Cell. 2006 Nov 17;127(4):803-15. PubMed PMID: 17110338.
Storage Store the antibody at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
State:
Liquid purified Ig
Aff - Purified
Species Reactivity
Species reactivity (expected):
Chimpanzee.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody is directed against AHA1 protein.

Accessory Products

Proteins and/or Positive Controls

Proteins for AHA1 / AHSA1 (6 products)

Catalog No. Species Pres. Purity   Source  

AHA1 / AHSA1

AHA1 / AHSA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 (19-337)

Activator of Hsp90 ATPase-1 AHA1 (15% SDS PAGE) Human Purified > 95 % > / = 95% by SDS PAGE E. coli
0.5 mg / €750.00
  OriGene Technologies GmbH

AHA1 / AHSA1 (19-337)

Activator of Hsp90 ATPase-1 AHA1 (15% SDS PAGE) Human Purified > 95 % > / = 95% by SDS PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

AHA1 / AHSA1

AHA1 / AHSA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AHA1 / AHSA1

AHA1 / AHSA1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AHA1 / AHSA1

SDS-Page: AHSA1 Protein [NBC1-18368] - AHA 1, 36.1 kDa (320 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
  Novus Biologicals Inc.

Positive controls for AHA1 / AHSA1 (3 products)

Catalog No. Species Pres. Purity   Source  

AHSA1 293T Cell Transient Overexpression Lysate(Denatured)

AHSA1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

AHSA1 Lysate

Western Blot: AHSA1 Lysate [NBL1-07404] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AHSA1 Protein
  Novus Biologicals Inc.

AHSA1 overexpression lysate

AHSA1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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