TA319559 AKT1 / PKB pThr308 antibody

Mouse monoclonal Akt phospho pT308 antibody

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0.1 mg / €515.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Monkey, Mouse, Rat AKT1 / PKB 18F3.H11

TA319559

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  • TA319559

Product Description for AKT1 / PKB

Mouse anti Human, Monkey, Mouse, Rat AKT1 / PKB 18F3.H11.
Properties: pThr308
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for AKT1 / PKB

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Presentation Purified
Reactivity Hu, Mky, Ms, Rt
Applications E, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 18F3.H11
Host Mouse
Isotype IgG1
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P31749
Immunogen:
Synthetic peptide corresponding to residues surrounding Thr308 of Human AKT1 protein
GeneID:
207
Property pThr308
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application ELISA: 1/20,000.
Western Blot: 1/500-1/3,000. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. 
Immunohistochemistry: 20 µg/ml. Use formalin-fixed paraffin-embedded sections.
Immunoprecipitation.
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Concentration 1.48 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Lawlor MA, Alessi DR. PKB/Akt: a key mediator of cell proliferation, survival and insulin responses? J Cell Sci. 2001 Aug;114(Pt 16):2903-10. PubMed PMID: 11686294.
  2. Alessi DR. Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture. Biochem Soc Trans. 2001 May;29(Pt 2):1-14. PubMed PMID: 11356119.
  3. Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4171-5. PubMed PMID: 1851997. (Free PMC Article available)
  4. Staal SP. Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma. Proc Natl Acad Sci U S A. 1987 Jul;84(14):5034-7. PubMed PMID: 3037531. (Free PMC Article available)
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid purified IgG fraction from tissue culture supernatant
Purified
Species Reactivity
Species reactivity (expected):
Monkey, Rat.
Species reactivity (tested):
Human, Mouse.
Specificity
Specificity:
This antibody is specific for Human and Mouse AKT protein phosphorylated at Thr308. 
This phospho-specific monoclonal antibody reacts with human and mouse AKT pThr308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. 
A BLAST analysis was used to suggest cross-reactivity with AKT pThr308 from most vertebrate species sources based on 100% homology with the immunizing sequence. 
Cross-reactivity with AKT from other sources has not been determined.  
Cross-reactivity with AKT2 and AKT3 will likely occur.

Accessory Products

Proteins and/or Positive Controls

Proteins for AKT1 / PKB (48 products)

Catalog No. Species Pres. Purity   Source  

AKT1 / PKB (transcript variant 3)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 2)

AKT1 / PKB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AKT1 / PKB (transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AKT1 / PKB (C-term MYC/DDK tag, transcript variant 1)

AKT1 / PKB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >95.0% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (active kinase)

AKT1 / PKB Human Purified >90.0% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB (Substrate)

AKT1 / PKB Human Purified > 98 % pure by HPLC analysis.
0.5 mg / €305.00
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
0.25 mg / €1,070.00
  OriGene Technologies GmbH

AKT1 / PKB (1-480, His-tag)

AKT1 / PKB Human Purified > 90 % by SDS - PAGE.
50 µg / €320.00
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

Precursor-Protein structure and amino acid sequence Human Purified > 95 % Greater than 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination. AKT1 is purified by proprietary chromatographic techniques. SF9 cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human Purified > 95 % Greater than 90% as determined by SDS-PAGE Insect cells
  OriGene Technologies GmbH

AKT1 / PKB

AKT1 / PKB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human Purified
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB

AKT1 / PKB Human
  Abnova Taiwan Corp.

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis-GST tagged Human Purified > 95 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis-GST tagged Human Purified > 95 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis-GST tagged Human Purified > 95 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis tagged Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis tagged Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

AKT1 / PKB (1-480)

Human AKT1 (RAC-alpha serine/threonine-protein kinase) recombinant protein 6xHis tagged Human Purified > 90 % 10% SDS-PAGE stained with Coomassie Blue (CB), immunobloting with anti-6xHis (WB) serum and peptide fingerprinting by MALDI-TOF-TOF mass spectrometry E. coli
  Protein Alternatives

Positive controls for AKT1 / PKB (1 products)

Catalog No. Species Pres. Purity   Source  

AKT1 Lysate

Western Blot: AKT1 Lysate [NBL1-07441] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AKT1 Protein
  Novus Biologicals Inc.
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