AP20103AF-N Alcohol Oxidase antibody

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10 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Candida Alcohol Oxidase

AP20103AF-N

Product Description for Alcohol Oxidase

Rabbit anti Candida Alcohol Oxidase.
Presentation: Azide Free
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Alcohol Oxidase

Product Category Primary Antibodies
Quantity 10 mg
Synonyms AOD1, AOX, MOX, Methanol Oxidase
Presentation Azide Free
Reactivity Candida
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q00922
Immunogen:
Alcohol dehydrogenase isolated and purified from Candida boidinii.
Freund's complete adjuvant is used in the first step of the immunization procedure.
Application Indirect Immunofluorescence.
ELISA.
Dot blot.
Western blot.
Recommended Dilutions:
Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/80,000.
Background Alcohol Oxidase (AOX) is a homooctameric flavoprotein consisting of eight identical subunits of ~74 kD, each containing a flavin adenine dinucleotide molecule (FAD) as a prosthetic group (van der Klei et al., 1991). The protein catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of certain yeasts including Pichia pastoris, Hansenula polymorpha, and Candida boidinii (van der Klei et al., 1991). AOX is normally localized in the matrix of single membrane-bound organelles called peroxisomes. During methanol growth, the peroxisomes also contain large amounts of dihydroxyacetone synthase, the first enzyme in the methanol assimilatory pathway, and catalase (CAT), which converts the hydrogen peroxide generated by oxidases such as AOX into water and oxygen (Veenhuis and Harder, 1991). As a result, peroxisomes, which are small and few in number in glucose-grown cells, are massively induced in methanol-grown cells (Veenhuis and Harder, 1991).
Concentration 10.0 mg/ml
Storage Store the antibody lyophilized at 2-8°C.
Reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Buffer System:
PBS, pH 7.2 without preservatives and foreign proteins.
State:
Lyophilized IgG fraction.
Azide Free
Reconstitution:
Restore by adding 1.0 ml of sterile distilled water; Prepare working dilutions by adding sterile phosphate buffered saline (PBS, ph 7.2) and preferably use the same day.
Specificity
Specificity:
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Recognizes Alcohol Oxidase.
Species:
Candida boidinii.

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