AP05001HR-N Alkaline phosphatase antibody

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Rabbit anti Escherichia coli Alkaline phosphatase


Product Description for Alkaline phosphatase

Rabbit anti Escherichia coli Alkaline phosphatase.
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Alkaline phosphatase

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms JW0374, b0383, phoA
Presentation HRP
Reactivity E. coli
Applications C, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Full length E. coli Alkaline Phosphatase
Application ELISA (1/3000-1/15000).
Western blot.
Immunohistochemistry on Frozen Sections.
Background Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
Concentration 10.0 mg/ml
General Readings
  1. Bradshaw RA, Cancedda F, Ericsson LH, Neumann PA, Piccoli SP, Schlesinger MJ, et al. Amino acid sequence of Escherichia coli alkaline phosphatase. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3473-7. PubMed PMID: 7022451. (Free PMC Article available)
  2. Wyckoff HW. (1987) Structure of Escherichia coli alkaline phosphatase determined by X- ray diffraction. Phosphate Metabolism and Cellular Regulation in Microorganisms. Edited by: Torriani – Gorini, A., Rothman, FG., Silver, S., Wright, A. and Yagil, E. America Society for Microbiology, Washington, DC. pp. 118–126.
  3. Akiyama Y, Ito K. Folding and assembly of bacterial alkaline phosphatase in vitro and in vivo. J Biol Chem. 1993 Apr 15;268(11):8146-50. PubMed PMID: 8463326.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Ion Exchange Chromatography
Buffer System:
PBS, pH 7.2 containing 0.01% Gentamicin Sulphate as preservative and 1% BSA as stabilizer.
Liquid purified Ig fraction
This antibody detects bacterial Alkaline Phosphatase (BAP), a periplasmic enzyme which catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides. BAP can also dephosphorylate a number of proteins and alkaloids, and has an optimum pH of 8.0. BAP is encoded by the phoA gene, and forms a homodimer of two identical 47kD subunits.
It is thought that bacteria use BAP to create free phosphate groups for uptake and use. Other functions include dephosphorylating extracellular organic compounds for import into the cell.
Bacteria. Does not work with Mammals.
Gene ID 945041

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