SM3165P alpha + beta Tubulin (heterodimer) antibody

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0.1 mg / €240.00
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Mouse anti All Species alpha + beta Tubulin TU-10

Product Description for alpha + beta Tubulin

Mouse anti All Species alpha + beta Tubulin TU-10.
Properties: (heterodimer)
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for alpha + beta Tubulin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms alpha-tubulin, beta-tubulin, tubulin alpha, tubulin beta
Presentation Purified
Reactivity All Species
Applications ICC/IF, WB
Clonality Monoclonal
Clone TU-10
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Microtubule proteins from porcine brain
Property heterodimer
Isotype control SM13P
Application Western blot.
Recommended Dilution: 2 µg/µl.
Positive control: porcine brain lysate.
Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunocytochemistry.
Background The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Concentration 1.0 mg/ml
General Readings
  1. Cowan NJ, Dobner PR, Fuchs EV, Cleveland D.W.: Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions. Mol Cell Biol. 1983 Oct;3(10):1738-45.
  2. Linhartova I, Draberova E, Viklicky V, Draber P.: Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells. FEBS Lett. 1993 Mar 29;320 (1):79-82.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Precipitation methods and size-exclusion chromatography
Buffer System:
Tris buffered saline (TBS), pH~8.0
Preservatives:
15 mM Sodium Azide
Purity:
> 95% (by SDS-PAGE).
State:
Liquid purified Ig fractionn (> 95% pure by SDS-PAGE)
Purified
Species Reactivity
Species reactivity (tested):
All (recognized epitope conserved within all species)
Specificity
Specificity:
The antibody TU-10 recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules. Alpha- (50 kDa) and beta- (50 kDa) tubulins form Tubulin heterodimer, a globular protein that polymerizes to form microtubules.
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