discontinued

AM39110PU-N alpha Tubulin / TUBA1A / TUBA3 antibody

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0.1 mg / €430.00

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Mouse anti Chicken, Human, Mouse, Rat alpha Tubulin / TUBA1A / TUBA3 DM1A

AM39110PU-N

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  • AM39110PU-N
  • AM39110PU-N

Product Description for alpha Tubulin / TUBA1A / TUBA3

Mouse anti Chicken, Human, Mouse, Rat alpha Tubulin / TUBA1A / TUBA3 DM1A.
Presentation: Purified
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for alpha Tubulin / TUBA1A / TUBA3

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-3 chain
Presentation Purified
Reactivity Chk, Hu, Ms, Rt
Applications ICC/IF, P, WB
Clonality Monoclonal
Clone DM1A
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02552
Immunogen:
Native chicken brain microtubules
GeneID:
5777
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blot: 0.01 µg/mL of this antibody detected α-tubulin in 10 µg of HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected α-tubulin in NIH/3T3, A431, and HeLa cells.
Immunohistochemistry on FFPE sections: A 1:10,000 dilution from a representative lot detected α-tubulin in normal human brain tissue, human brain cancer tissue, normal rat hippocampus tissue and normal rat cerebellum tissue.
Background Tubulin is one of the main components of the cytoskeleton. It is a heterodimeric structure consisting of interlocking alpha and beta chains. Like other cytoskeleton proteins, tubulin undergoes dynamic changes depending on the cellular context, which are mediated by binding and release of GDP/GTP. Tubulin proteins may also undergo post-translational modifications, and are essential to cellular transport and mitotic processes.
Concentration 1.0 mg/ml (lot-specific)
Protocols Western Blotting
1. Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on cell lysate and transfer the proteins to a PVDF membrane. Wash the PVDF membrane twice with water.
2. Block the blotted PVDF membrane in freshly prepared 5% BSA or milk with 0.05% Tween®-20 surfactant for 1 hour at room temperature with constant agitation.
3. Incubate the PVDF with the recommended dilution of the primary antibody, diluted in freshly prepared 5% BSA or milk for 1 hour at room temperature or overnight with agitation at 2-8°C.
4. Wash the PVDF 3 times with TBST.
5. Incubate the PVDF in the secondary reagent of choice in 5% milk for 1 hour with agitation at room temperature.
6. Wash the PVDF 3-5 times with TBST.
7. Visualize with enhanced chemiluminescence (ECL) method of choice.
General Readings
  1. Saugstad JA, Yang S, Pohl J, Hall RA, Conn PJ. Interaction between metabotropic glutamate receptor 7 and alpha tubulin. J Neurochem. 2002 Mar;80(6):980-8. PubMed PMID: 11953448. (Free PMC Article available, 5 images available)
  2. Erickson HP, O'Brien ET. Microtubule dynamic instability and GTP hydrolysis. Annu Rev Biophys Biomol Struct. 1992;21:145-66. PubMed PMID: 1525467.
Storage Store undiluted at 2-8°C.
Shelf life: One year from despatch.
Format
Purification:
Protein G chromatography
Buffer System:
0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
State:
Liquid purified IgG1 fraction
Purified
Specificity
Specificity:
The antibody reacts with Human and Mouse and Rat alpha-Tubulin.
Predicted to react with Chicken based on 100% sequence homology.

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