Product Description for alpha Tubulin / TUBA1A / TUBA3
Chicken anti Human, Mouse, Rat alpha Tubulin / TUBA1A / TUBA3.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.
Properties for alpha Tubulin / TUBA1A / TUBA3
| Product Category | Primary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.1 mg |
| Synonyms | Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-1A chain, Tubulin alpha-3 chain |
| Presentation | Aff - Purified |
| Reactivity | Hu, Ms, Rt |
| Applications | E, WB |
| Clonality | Polyclonal |
| Host | Chicken |
| Isotype | IgG |
| Shipping to | Europe, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies, Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Add. information | Blocking peptide available: blocking peptide (AP30046CP-N) |
| Application | ELISA. Western Blot: Tubulin antibody can be used for detection of Tubulin at 1 - 2 μg/ml. |
| Background | Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots. |
| General Readings |
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| Storage | Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch. |
| Format |
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| Specificity |
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Accessory Products
Proteins and/or Positive Controls
Proteins for alpha Tubulin / TUBA1A / TUBA3 (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP308669 |
alpha Tubulin / TUBA1A / TUBA3 |
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|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||||
| H00007846-P01-10 |
alpha Tubulin / TUBA1A / TUBA3 |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00007846-P01-25 |
alpha Tubulin / TUBA1A / TUBA3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00007846-Q01-10 |
alpha Tubulin / TUBA1A / TUBA3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00007846-Q01-25 |
alpha Tubulin / TUBA1A / TUBA3 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for alpha Tubulin / TUBA1A / TUBA3 (1 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-17427 |
alpha Tubulin Lysate |
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![Western Blot: alpha Tubulin Lysate [NBL1-17427] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for TUBA1A](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/alpha%20Tubulin%20Lysate-Western%20Blot-NBL1-17427-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for alpha Tubulin / TUBA1A / TUBA3 (8 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1300AP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
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|
|
Rabbit | AP | |||||
| OriGene Technologies GmbH | |||||||
| R1300B |
Chicken IgG / Chicken IgY (H+L chain) antibody |
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|
|
Rabbit | Biotin | |||||
| OriGene Technologies GmbH | |||||||
| R1300F |
Chicken IgG / Chicken IgY (H+L chain) antibody |
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|
|
Rabbit | FITC | F, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1300HRP |
Chicken IgG / Chicken IgY (H+L chain) antibody |
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|
|
Rabbit | HRP | |||||
| OriGene Technologies GmbH | |||||||
| R1300T |
Chicken IgG / Chicken IgY (H+L chain) antibody |
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|
|
Rabbit | TRITC | |||||
| OriGene Technologies GmbH | |||||||
| R1434C2 |
Chicken IgG / Chicken IgY (H+L chain), adsorbed antibody |
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|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1434C3 |
Chicken IgG (H&L) multi-species ads. antibody |
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|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1434C5 |
Chicken IgG / Chicken IgY (H+L chain, adsorbed) antibody |
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|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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