AM33343SU-S AMACR / RACE antibody

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0.1 ml / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Rabbit anti Human AMACR / RACE 13H4

AM33343SU-S

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  • AM33343SU-S

Product Description for AMACR / RACE

Rabbit anti Human AMACR / RACE 13H4.
Presentation: Supernatant
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for AMACR / RACE

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase, P504S
Presentation Supernatant
Reactivity Hu
Applications P, WB
Clonality Monoclonal
Clone 13H4
Host Rabbit
Isotype IgG
Molecular weight 54kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UHK6
Immunogen:
Full length Human recombinant AMACR protein.
GeneID:
23600
Application Western Blotting: 1/100-1/200 for 2 hours at RT. 
Immunohistochemistry on Formalin-Fixed Paraffin Sections: 1/50-1/100 for 30-60 min at RT.  
Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5 for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: HEK cells or Prostate Adenocarcinoma.
Background AMACR (P504S) is an essential enzyme in the beta-oxidation of branched-chain fatty acids. Recently, AMACR (P504S) was identified through cDNA library subtraction and microarrays in malignant prostate tissues.
High expression of AMACR (P504S) protein is found in prostatic adenocarcinoma but not in benign prostatic tissue by immunohistochemical staining in paraffin-embedded tissues. The expression of AMACR (P504S) is also detected in two premalignant lesions of the prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. Using AMACR (P504S) as a positive marker along with basal cell staining (34 βE12 or P63) as a negative marker could help to confirm the diagnosis of small focus of prostate carcinoma on needle biopsy.
General Readings
  1. Xu J, Stolk JA, Zhang X, Silva SJ, Houghton RL, Matsumura M, et al. Identification of differentially expressed genes in human prostate cancer using subtraction and microarray. Cancer Res. 2000 Mar 15;60(6):1677-82. PubMed PMID: 10749139.
  2. Jiang Z, Fanger GR, Woda BA, Banner BF, Algate P, Dresser K, et al. Expression of alpha-methylacyl-CoA racemase (P504s) in various malignant neoplasms and normal tissues: astudy of 761 cases. Hum Pathol. 2003 Aug;34(8):792-6. PubMed PMID: 14506641.
  3. Jiang Z, Woda BA, Rock KL, Xu Y, Savas L, Khan A, et al. P504S: a new molecular marker for the detection of prostate carcinoma. Am J Surg Pathol. 2001 Nov;25(11):1397-404. PubMed PMID: 11684956.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Preservatives:
0.05% Sodium Azide
State:
Liquid Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal Antibody recognizes a protein of 54kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. AMACR is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
Cellular LocalizationCytoplasmic.

Accessory Products

Proteins and/or Positive Controls

Proteins for AMACR / RACE (11 products)

Catalog No. Species Pres. Purity   Source  

AMACR / RACE (transcript variant 1)

AMACR / RACE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AMACR / RACE (full length, N-term HIS tag, transcript variant 2)

AMACR / RACE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human
  Abnova Taiwan Corp.

AMACR / RACE

AMACR / RACE Human in vitro transl.
  Abnova Taiwan Corp.

AMACR / RACE

AMACR / RACE Human in vitro transl.
  Abnova Taiwan Corp.

Positive controls for AMACR / RACE (5 products)

Catalog No. Species Pres. Purity   Source  

AMACR Lysate

Western Blot: AMACR Lysate [NBL1-07494] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AMACR Protein
  Novus Biologicals Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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