discontinued

AP09884PU-N AMACR / RACE (N-term) antibody

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Rabbit anti Human, Mouse, Rat AMACR / RACE

AP09884PU-N

Product Description for AMACR / RACE

Rabbit anti Human, Mouse, Rat AMACR / RACE.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for AMACR / RACE

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase, P504S
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UHK6
Immunogen:
Synthetic peptides
AA Sequence:
gaa vlr rlc krs dvl lep f r
GeneID:
23600
Property N-term
Application ELISA.
Western blot: > 1/500.
Immunoprecipitation: > 1/200.
Does not work well in Immunohistochemistry.
Background A mitochondrial and peroxisomal enzyme, Alpha-methyacyl-CoA racemase (AMCAR), an enzyme invloved in beta oxidation of branched chain fatty acods and bile salt intermidiates, and is recently identified as a neomarker for prostate cancer. The AMCAR is over expressed in prostate cancer. Several different isoforms have been reported that are produced either by extensinve alternatve splicing of 5 exons or by use of alternate initiation codons. Atleast 2 different transcripts each derived from the 5 exons have been reported, AMCAR I abd AMACR II. The AMCAQR I is the most abundant form and enclodes for a 382 amino acid protein (42kDa) with a PI of 6.0. The other isoform AMACR II has an alternative fifth exon that exhibit significnat homolgy to fumerate hydratase and encoes a 288 amino acid protein with a molecular weight of 32 kDa, PI 9.6. Several other variants of IA and IIA isoforms are charcterized recently (1). The variant lack exon lack exon 3 are designated as IB and IIB. In prostate tumor tissues that overexpressed AMACR, both the A and B forms are over-expressed. The predominant isoform AMCAR IA also has a peroxisomal targetting signal peptide (PTS1), while other varinats are basic in PI and lack the PTS1.
Carcinomas of the transition zone (TZ) constitute approximately 20% of all prostate cancers. The TZ is the site of origin of grade 1 and grade 2 cancers, the most well-differentiated of the Gleason grade tumors, as well as for benign prostatic hyperplasia (BPH). AMACR has been proposed as a new molecular marker for prostate cancer, because the enzyme is reportedly overexpressed in high-grade dysplasias, also termed prostatic intraepithelial neoplasia, a purported precursor of prostatic carcinoma, and in all grades of prostatic carcinoma of the peripheral zone (3). Small interference RNA (siRNA) against AMACR, but not the control inverted siRNA, reduced the expression of AMACR and significantly impaired proliferation of the androgen-responsive PCa cell line LAPC-4 (2) suggesting that AMACR is essential for optimal growth of PCa cells in vitro and that this enzyme has the potential to be a complementary target with androgen ablation in PCa treatment.
Concentration 0.75 mg/ml
General Readings
  1. Mubiru JN, Shen-Ong GL, Valente AJ, Troyer DA. Gene. 2004 Feb 18;327(1):89-98.
  2. Zha S, Ferdinandusse S, Denis S, Wanders RJ, Ewing CM, Luo J, De Marzo AM, Isaacs WB. Cancer Res. 2003 Nov 1;63:7365-76.
  3. Leav I, McNeal JE, Ho SM, Jiang Z. Alpha-methylacyl-CoA racemase (P504S) expression in evolving carcinomas within benign prostatic hyperplasia and in cancers of the transition zone. Hum Pathol. 2003 Mar;34(3):228-33.
Storage Store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
Stabilization buffer
State:
Liquid purified IgG fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, mouse, rat
Specificity
Specificity:
This antibody detects AMACR at N-term.
Gene ID 23600

Accessory Products

Proteins and/or Positive Controls

Proteins for AMACR / RACE (11 products)

Catalog No. Species Pres. Purity   Source  

AMACR / RACE (transcript variant 1)

AMACR / RACE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

AMACR / RACE (full length, N-term HIS tag, transcript variant 2)

AMACR / RACE Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human Purified > 90 % E. coli
  OriGene Technologies GmbH

AMACR / RACE

AMACR / RACE Human
  Abnova Taiwan Corp.

AMACR / RACE

AMACR / RACE Human in vitro transl.
  Abnova Taiwan Corp.

AMACR / RACE

AMACR / RACE Human in vitro transl.
  Abnova Taiwan Corp.

Positive controls for AMACR / RACE (5 products)

Catalog No. Species Pres. Purity   Source  

AMACR Lysate

Western Blot: AMACR Lysate [NBL1-07494] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AMACR Protein
  Novus Biologicals Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.

AMACR overexpression lysate

AMACR overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.
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