discontinued

AP01882PU-N AMPK1 / AMPK2 pThr174/172 antibody

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0.1 mg / €380.00

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Rabbit anti Human, Mouse, Rat AMPK1 / AMPK2

AP01882PU-N

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  • AP01882PU-N

Product Description for AMPK1 / AMPK2

Rabbit anti Human, Mouse, Rat AMPK1 / AMPK2.
Properties: pThr174/172
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for AMPK1 / AMPK2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-1 chain, AMPK alpha-2 chain, PRKAA1, PRKAA2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight ~ 62 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Property pThr174/172
Application Western Blot: 1/500-1/1000.
Immunohistochemistry on paraffin sections: 1/50-1/200.
Background Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunits, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKα has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the α subunits
Concentration 1.0 mg/ml
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific immunogen.
Buffer System:
Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
State:
Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
Aff - Purified
Species Reactivity
Species reactivity (expected):
Mouse and Rat.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody detects endogenous levels of AMPK alpha1/2 protein when phosphorylated at Thr174 or Thr172.

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