discontinued

NB110-62079 AMPK1 / AMPK2 pThr172 antibody

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0.1 ml / €390.00

Quick Overview

Rabbit anti Human, Mouse, Rat AMPK1 / AMPK2

Product Description for AMPK1 / AMPK2

Rabbit anti Human, Mouse, Rat AMPK1 / AMPK2.
Properties: pThr172
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for AMPK1 / AMPK2

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-1 chain, AMPK alpha-2 chain, PRKAA1, PRKAA2
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK that contains threonine 172. The sequence is conserved in human, mouse and rat.
Property pThr172
Add. information Research Areas: Cancer
Application Western Blot, This antibody is useful in Western Blot., Western Blot 1:1000,
Background AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostatis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Concentration 1 mg/ml
General Readings
  1. McCullough LD, Zeng Z, Li H, Landree LE, McFadden J, Ronnett GV. Pharmacological inhibition of AMP-activated protein kinase provides neuroprotection in stroke. J Biol Chem. 2005 May 27;280(21):20493-502. Epub 2005 Mar 16. PubMed PMID: 15772080.
  2. Jørgensen SB, Nielsen JN, Birk JB, Olsen GS, Viollet B, Andreelli F, et al. The alpha2-5'AMP-activated protein kinase is a site 2 glycogen synthase kinase in skeletal muscle and is responsive to glucose loading. Diabetes. 2004 Dec;53(12):3074-81. PubMed PMID: 15561936.
  3. Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, et al. The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 25. PubMed PMID: 14985505. (Free PMC Article available, 6 images available)
  4. Zou MH, Kirkpatrick SS, Davis BJ, Nelson JS, Wiles WG, Schlattner U, et al. Activation of the AMP-activated protein kinase by the anti-diabetic drug metformin in vivo. Role of mitochondrial reactive nitrogen species. J Biol Chem. 2004 Oct 15;279(42):43940-51. Epub 2004 Jul 19. PubMed PMID: 15265871.
  5. Woods A, Vertommen D, Neumann D, Turk R, Bayliss J, Schlattner U, et al. Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis. J Biol Chem. 2003 Aug 1;278(31):28434-42. Epub 2003 May 21. PubMed PMID: 12764152.
  6. Yin W, Mu J, Birnbaum MJ. Role of AMP-activated protein kinase in cyclic AMP-dependent lipolysis In 3T3-L1 adipocytes. J Biol Chem. 2003 Oct 31;278(44):43074-80. Epub 2003 Aug 25. PubMed PMID: 12941946.
  7. Hawley SA, Gadalla AE, Olsen GS, Hardie DG. The antidiabetic drug metformin activates the AMP-activated protein kinase cascade via an adenine nucleotide-independent mechanism. Diabetes. 2002 Aug;51(8):2420-5. PubMed PMID: 12145153.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Affinity purified
State:
Aff - Purified
Specificity
Specificity:
Human, mouse and rat.
Species:
Human, Mouse, Rat

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