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BM2568 Amyloid Beta (17-24) antibody

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Mouse anti Human Amyloid Beta (17-24) 4G8

BM2568

Product Description for Amyloid Beta (17-24)

Mouse anti Human Amyloid Beta (17-24) 4G8.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Amyloid Beta (17-24)

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Purified
Reactivity Hu
Applications E, IP, P, WB
Clonality Monoclonal
Clone 4G8
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control AM03110PU-N, SM12P
Application ELISA
Western blot
Immunoprecipitation
Immunohistochemistry: Formalin-fixed paraffin-embedded tissue sections.
Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
1.Formic acid (70%) for 10-30 minutes at room temperature.
2.HIER (heat Induced Epitope Retrieval)
Concentration ~1mg/ml (OD 280 nm)
General Readings
  1. Kimura, N., et al., (2005), Age-related changes of intracellular A-beta in cynomolgus monkey brains, Neuropatho. Appl. Neurobiol., 31(2), 170-180.
  2. Klyubin, I., et al., (2005), Anyloid beta protein immunotherapy neutralizes A-beta oligomers that disrupt synaptic plasticity in vivo, Nat. Med., 11(5): 556-561.
  3. Iijima, K., et al., (2004), Dissecting pathological effects of human A-beta 40 and A-beta 42 in Drosophila: a potential model for Alzheimer's disease, PNAS, 101(17), 6623-6628.
  4. Li M, Pisalyaput K, Galvan M, Tenner AJ. Macrophage colony stimulatory factor and interferon-gamma trigger distinct mechanisms for augmentation of beta-amyloid-induced microglia-mediated neurotoxicity. J Neurochem. 2004 Nov;91(3):623-33. PubMed PMID: 15485493.
  5. Poduslo JF, Curran GL, Peterson JA, McCormick DJ, Fauq AH, Khan MA, et al. Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer's disease amyloid plaques. Biochemistry. 2004 May 25;43(20):6064-75. PubMed PMID: 15147190. (Free PMC Article available, 10 images available)
  6. Venezia V, Russo C, Repetto E, Salis S, Dolcini V, Genova F, et al. Apoptotic cell death influences the signaling activity of the amyloid precursor protein through ShcA and Grb2 adaptor proteins in neuroblastoma SH-SY5Y cells. J Neurochem. 2004 Sep;90(6):1359-70. PubMed PMID: 15341520.
  7. Kim, K.S., et al., (1988), Production and characterization of monoclonal antibodies reactive to synthetic cerebrovascular amyloid peptide, Neuroscience Research Communications, 2: 121-130.
  8. Kim, K.S., et al., (1988), Neuroscience Research Communications, 7, 113.
Storage Aliquot and store the antibody at -20 to -70°C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A chromatography
Buffer System:
PBS containing no preservative
State:
Liquid
Purified
Specificity
Specificity:
Reactive to amino acid residues 17-24 of human Amyloid Beta peptide. Epitope is within amino acids 18-22 of beta amyloid peptide (VFFAE). Reacts to the abnormally processed isoforms as well as precursor forms.

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