NB100-1762 AOF2 antibody

See related secondary antibodies

Search for all "AOF2"

0.1 ml / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human AOF2 1B2E5

NB100-1762

More Views

  • NB100-1762
  • NB100-1762

Product Description for AOF2

Mouse anti Human AOF2 1B2E5.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for AOF2

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1
Presentation Ascites
Reactivity Hu
Applications C, E, P, WB
Clonality Monoclonal
Clone 1B2E5
Host Mouse
Isotype IgG
Molecular weight 93 kDa
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O60341
Immunogen:
Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]
GeneID:
23028
Add. information Research Areas: DNA Repair
Application Western Blot: 1:500-1:2000. 
Simple Western: 1:500. 
Chromatin Immunoprecipitation. 
ELISA: 1:10000. 
Immunohistochemistry: 1:200-1:1000. 
Immunohistochemistry-Paraffin: 1:200-1:1000.
Note: This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Background The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.
Concentration This product is unpurified. The exact concentration of antibody is not quantifiable.
General Readings
  1. Shi YJ, Matson C, Lan F, Iwase S, Baba T, Shi Y. Regulation of LSD1 histone demethylase activity by its associated factors. Mol Cell. 2005 Sep 16;19(6):857-64. PubMed PMID: 16140033.
  2. Metzger E, Wissmann M, Yin N, Müller JM, Schneider R, Peters AH, et al. LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription. Nature. 2005 Sep 15;437(7057):436-9. Epub 2005 Aug 3. PubMed PMID: 16079795.
Storage Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.
Format
Purification:
Ascites
Buffer System:
0.03% Sodium Azide
Purity:
protein A purified
State:
Ascites
Specificity
Specificity:
LSD1 (1B2E5)
Species:
Human, Mouse, Primate.
Gene ID 23028

Accessory Products

Proteins and/or Positive Controls

Proteins for AOF2 (8 products)

Catalog No. Species Pres. Purity   Source  

AOF2 (transcript variant 1)

AOF2 Human > 80 %
Preparation: or Add: Recombint proteins was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

AOF2

AOF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

AOF2

AOF2 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

Positive controls for AOF2 (4 products)

Catalog No. Species Pres. Purity   Source  

AOF2 293T Cell Transient Overexpression Lysate(Denatured)

AOF2 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

KDM1A overexpression lysate

KDM1A overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.

KDM1A overexpression lysate

KDM1A overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.

LSD1 Lysate

Western Blot: LSD1 Lysate [NBL1-07573] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KDM1A Protein
0.1 mg / €400.00
  Novus Biologicals Inc.
  • LinkedIn