AM09117PU-N APAAP Complex antibody

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Mouse anti Mouse APAAP Complex 27/21


Product Description for APAAP Complex

Mouse anti Mouse APAAP Complex 27/21.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for APAAP Complex

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Ms
Applications E, ICC/IF, P, WB
Clonality Monoclonal
Clone 27/21
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Alkaline Phosphatase from Calf Intestine
Isotype control AM03095PU-N
Application Enzyme anti-Enzyme immunocomplex for use in Immunoassays for the detection of Mouse IgG, e.g. ELISA, Immunohistochemistry, Immunocytochemistry, Western blot, where endogenous peroxidase activity might cause background staining.
APAAP complex usually gives higher sensitivity results than peroxidase conjugate complexes.
Not recommended for intestinal tissue sections or extracts (presence of endogenous Alkaline Phosphatase activity, which cannot be blocked by e.g. levamisole).
Working Dilution: 1/50 for Immunohistochemistry, dilute e.g. with PBS, pH 7.4
Incubation Time: 30 min at RT.
General Readings
  1. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, et al. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219-29. PubMed PMID: 6198355.
  2. Lansdorp PM, Wognum AW, Zeijlemaker WP. Stepwise amplified immunoenzyme staining techniques for the detection of monoclonal antibodies and antigens. Methods Enzymol. 1986;121:855-67. PubMed PMID: 3523140.
  3. Mason, D.Y., Cordell, J.L., Abdulasiz, Z., Naiem, M. and Bordenave, G.: Preparation of peroxidase:antiperoxidase (PAP) complexes for immunohistological labelling of monoclonal antibodies. J. Histochem. Cytochem. 30, 114-1122. (1982)
  4. Naser, W.L.: Single incubation multilayer immunotechnique. J. Immunol. Methods 129, 151-157. (1990)
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Ion Exchange Chromatography complexed with Alkaline Phosphatase
Buffer System:
PBS, pH 7.4
Liquid purified IgG fraction
APAAP Complex

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