AP22589PU-N Apolipoprotein A I / APO AI antibody

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50 µg / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Goat anti Mouse Apolipoprotein A I / APO AI

AP22589PU-N

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  • AP22589PU-N

Product Description for Apolipoprotein A I / APO AI

Goat anti Mouse Apolipoprotein A I / APO AI.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Apolipoprotein A I / APO AI

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms APOA1, Apo-AI, ApoA-I, ApoAI
Presentation Purified
Reactivity Ms
Applications E, IP, P, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02647
Immunogen:
Apolipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification
GeneID:
335
Application ELISA: 1/5000 - 1/10000.
Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Immunoprecipitation.
Western Blot: 1/500 - 1/1000.
Background APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally. Variation in the latter step, in addition to modifications leading to so-called isoforms, is responsible for some of the polymorphism observed. APOA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the C-III gene is transcribed convergently in relation to A-I. Defects in the apolipoprotein A-1 gene are associated with HDL deficiency and Tangier disease.
Concentration 1 mg/ml
Storage Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. For extended storage, mix an equal volume of glycerol.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide
State:
Liquid Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Mouse
Specificity
Specificity:
Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross- reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible.
Gene ID 335

Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein A I / APO AI (17 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €139.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Hamster Purified Syrian hamster plasma, high density lipoproteins Plasma
0.1 mg / €1,680.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 90 % Plasma
0.5 mg / €340.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 99 % pure by SDS-PAGE. 
Purification: The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated Ethanol extraction.
The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted.
Plasma
1 mg / €360.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 95 % pure by SDS-PAGE (Column Chromatography). Plasma
0.5 mg / €250.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Mouse Purified Purified from Swiss-Webster Mouse high density lipoprotein plasma fraction by gel-sieving- and Ion Exchange Column Chromatography Plasma
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

SDS-Page: Apolipoprotein A I Protein [NBP1-30197] - Apolipoprotein A-1, 30.3 kDa (264aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for Apolipoprotein A I / APO AI (2 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I (APO AI) Lysate(Denatured)

Apolipoprotein A I (APO AI) Lysate(Denatured)
  Abnova Taiwan Corp.

Apolipoprotein A I Lysate

Western Blot: Apolipoprotein A I Lysate [NBL1-07609] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for APOA1 Protein
  Novus Biologicals Inc.
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