R1029P Apolipoprotein A I / APO AI antibody

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1 mg / €1,180.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Goat anti Human Apolipoprotein A I / APO AI

R1029P

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  • R1029P

Product Description for Apolipoprotein A I / APO AI

Goat anti Human Apolipoprotein A I / APO AI.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Apolipoprotein A I / APO AI

Product Category Primary Antibodies
Target Category
Quantity 1 mg
Synonyms APOA1, Apo-AI, ApoA-I, ApoAI
Presentation Purified
Reactivity Hu
Applications C, E, IP, P, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02647
Immunogen:
Apolipoprotein Type A-I was isolated from Human plasma by density gradient centrifugation followed by HPLC purification.
GeneID:
335
Application Anti-Apolipoprotein antibodies have been used for Indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive qualitative analysis.
Recommended Dilutions:
ELISA: 1/10,000-1/20,000.
Western blot: 1/1,000-1/2,000.
Immunoprecipitation: 1/100.
Immunohistochemistry: 1/50-1/200.
Background Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Bernthaler P, Epping K, Schmitz G, Deplazes P, Brehm K. Molecular characterization of EmABP, an apolipoprotein A-I binding protein secreted by the Echinococcus multilocularis metacestode. Infect Immun. 2009 Dec;77(12):5564-71. doi: 10.1128/IAI.00653-09. Epub 2009 Oct 5. PubMed PMID: 19805524. (Free PMC Article available, 5 images available)
Storage Store vial at 2-8°C prior to opening.
This product is stable at 2-8°C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities.
Buffer System:
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Preservatives:
0.01% Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
Typically less than 1% cross reactivity against other types of Apolipoprotein was detected by ELISA against purified standards.
This antibody reacts with Human Apolipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J Apolipoproteins.
Non-specific cross reaction of anti-Apolipoprotein antibodies with other Human serum proteins is negligible.
Gene ID 335
FocusOn and Reviews
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Accessory Products

Proteins and/or Positive Controls

Proteins for Apolipoprotein A I / APO AI (17 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €139.00
  OriGene Technologies, Inc.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Hamster Purified Syrian hamster plasma, high density lipoproteins Plasma
0.1 mg / €1,680.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €750.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI (25-267, His-tag)

Apolipoprotein A I / APO AI Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human > 97 % pure by SDS-PAGE gel and HPLC analyses. E. coli
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 90 % Plasma
0.5 mg / €340.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 99 % pure by SDS-PAGE. 
Purification: The protein was isolated by ultracentrifugal flotation between densities 1.063-1.21 g/ml, lyophilized then subjected to repeated Ethanol extraction.
The precipitated protein was dried under nitrogen then dissolved in 6 M Guanidine Hydrochloride -25 mM DTT. The product was then subjected to Sephacryl S200 filtration and eluted.
Plasma
1 mg / €360.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human Purified > 95 % pure by SDS-PAGE (Column Chromatography). Plasma
0.5 mg / €250.00
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Mouse Purified Purified from Swiss-Webster Mouse high density lipoprotein plasma fraction by gel-sieving- and Ion Exchange Column Chromatography Plasma
  OriGene Technologies GmbH

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

Apolipoprotein A I / APO AI Human
  Abnova Taiwan Corp.

Apolipoprotein A I / APO AI

SDS-Page: Apolipoprotein A I Protein [NBP1-30197] - Apolipoprotein A-1, 30.3 kDa (264aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for Apolipoprotein A I / APO AI (2 products)

Catalog No. Species Pres. Purity   Source  

Apolipoprotein A I (APO AI) Lysate(Denatured)

Apolipoprotein A I (APO AI) Lysate(Denatured)
  Abnova Taiwan Corp.

Apolipoprotein A I Lysate

Western Blot: Apolipoprotein A I Lysate [NBL1-07609] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for APOA1 Protein
  Novus Biologicals Inc.
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