AM26228BT-N Apoptotic Neutrophils antibody

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Mouse anti Human Apoptotic Neutrophils BOB93


Product Description for Apoptotic Neutrophils

Mouse anti Human Apoptotic Neutrophils BOB93.
Presentation: Biotin
Product is tested for Western blot / Immunoblot.

Properties for Apoptotic Neutrophils

Product Category Primary Antibodies
Quantity 50 µg
Presentation Biotin
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone BOB93
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM10B (for use in human samples)
Application Flow cytometry: The typical starting working dilution is 1:10.
Western blot: The typical starting working dilution is 1:10.
Background Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the body's defense against infectious agents, uncontrolled release of its toxic substances may inflict "friendly fire" damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis.
Concentration 0.1 mg/ml
General Readings
  1. Hart SP, Jackson C, Kremmel LM, McNeill MS, Jersmann H, Alexander KM, et al. Specific binding of an antigen-antibody complex to apoptotic human neutrophils. Am J Pathol. 2003 Mar;162(3):1011-8. PubMed PMID: 12598333. (Free PMC Article available, 7 images available)

Store at 2 - 8 °C.
Shelf life: one year from despatch.

Protein G
Buffer System:
0.02% sodium azide
0.1% bovine serum albumin
Liquid 0.2 µm filtered Ig fraction
Species Reactivity
Species reactivity (tested):
The monoclonal antibody BOB93 binds to the surface of apoptotic neutrophils.
Binding of the monoclonal antibody BOB93 to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for BOB93, and BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognised by BOB93 may act as a "molecular bridge" between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognised by BOB93, which does not bind to other apoptotic leukocytes.

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