NB500-120 Arginine (mono and dimethyl) antibody

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Mouse anti All Species, Human, Mammalia, Mouse, Rat Arginine 7E6

Product Description for Arginine

Mouse anti All Species, Human, Mammalia, Mouse, Rat Arginine 7E6.
Properties: (mono and dimethyl)
Presentation: Ascites
Product is tested for ChIP assay, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Arginine

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Ascites
Reactivity All Species, Hu, Mam, Ms, Rt
Applications ChIP, E, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone 7E6
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Asymmetrical NG/NG-dimethyl arginine
Property mono and dimethyl
Add. information Reconstitute in 200 ul of distilled water.
Application chromatin immunoprecipitation, ELISA, immunofluorescence, immunohistochemistry, immunoprecipitation, Western Blot, This antibody is useful for Chromatin Immunoprecipitation, ELISA , Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Western Blot , chromatin immunoprecipitation 3ul for 4x10 to the 6th cells, ELISA , immunohistochemistry 1:50 - 1:800, immunofluorescence , immunoprecipitation , Western Blot 1:500 - 1:5000
Background This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognizes both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.
General Readings
  1. Duong FH, Filipowicz M, Tripodi M, La Monica N, Heim MH. Hepatitis C virus inhibits interferon signaling through up-regulation of protein phosphatase 2A. Gastroenterology. 2004 Jan;126(1):263-77. PubMed PMID: 14699505.
  2. Fabbrizio E, El Messaoudi S, Polanowska J, Paul C, Cook JR, Lee JH, et al. Negative regulation of transcription by the type II arginine methyltransferase PRMT5. EMBO Rep. 2002 Jul;3(7):641-5. PubMed PMID: 12101096. (Free PMC Article available, 4 images available)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
No Preservative
This reacts with free and bound NG-NG-dimethyl arginine and cross reacts with free and bound NG-monomethyl arginine. It does not recognize unmodified free or bound arginine.
All Species, Mammal

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