discontinued

NB110-74766 ASIC1 antibody

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0.1 ml / €350.00

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Rabbit anti Human, Mouse, Rat ASIC1

NB110-74766

Product Description for ASIC1

Rabbit anti Human, Mouse, Rat ASIC1.
Presentation: Serum
Product is tested for Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for ASIC1

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Serum
Reactivity Hu, Ms, Rt
Applications C, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
A synthetic peptide as a part of human and rat amiloride-sensitive cation channel 2, neuronal (ASIC1) conjugated to immunogenic carrier protein was used as the immunogen.
Application immunohistochemistry , Western Blot 1:300 to 1:4000
Background This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown.; FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+).; SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons.; TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.;
General Readings
  1. Waldmann R, et al. Nature 386:173-177(1997).
  2. Chen C.-C, et al. Proc. Natl. Acad. Sci. U.S.A. 95:10240-10245(1998).
  3. Baessler E.-L, et al. J. Biol. Chem. 276:33782-33787(2001).
  4. Ugawa S, et al. NeuroReport 12:2865-2869(2001).
  5. Bassilana F, et al. J. Biol. Chem. 272:28819-28822(1997).
  6. Escoubas P, et al. J. Biol. Chem. 275:25116-25121(2000).
  7. Askwith C.C, et al. Neuron 26:133-141(2000).
  8. Voilley N, et al. J. Neurosci. 21:8026-8033(2001).
  9. Babini E, et al. J. Biol. Chem. 277:41597-41603(2002).
  10. Coric T, et al. J. Biol. Chem. 278:45240-45247(2003).
Storage Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1)
Format
Purification:
Whole antisera
Buffer System:
Lyophilised in PBS
Purity:
Whole antisera
State:
Serum
Specificity
Species:
Human and rat. Other species not yet tested.

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