NB100-94194 ASK1 antibody

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Rabbit anti Human, Mouse ASK1

Product Description for ASK1

Rabbit anti Human, Mouse ASK1.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for ASK1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu, Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Rabbit anti-ASK1 polyclonal antibody was raised against a peptide corresponding to amino acids 1356 to 1375 of human ASK1 (1). This sequence is different from that of mouse by last two amino acids (3).
Application Western Blot, This polyclonal antibody can be used for detection of ASK1 by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate from SW1353 can be used as positive control and a 155 kDa band can be detected., Western Blot 1:500-1:1000,
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Storage Store at 4C. Do not freeze.
IgG purified
Buffer System:
0.02% Sodium Azide
IgG purified
Human, Mouse

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