NB500-113 Asymetric Dimethylarginine (ADMA) antibody

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Mouse anti All Species Asymetric Dimethylarginine (ADMA) 21C7


Product Description for Asymetric Dimethylarginine (ADMA)

Mouse anti All Species Asymetric Dimethylarginine (ADMA) 21C7.
Presentation: Ascites
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for Asymetric Dimethylarginine (ADMA)

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms Asymetric Dimethyl Arginine
Presentation Ascites
Reactivity All Species
Applications P, WB
Clonality Monoclonal
Clone 21C7
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Asymmetric NG-NG-dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)
Add. information Reconstitute in 200 ul of distilled water.
Research Areas: DNA Repair
Application chromatin immunoprecipitation, immunohistochemistry, immunoprecipitation, Western Blot, ChIP: Use 5ul for 4x106 cells. IHC: Use at a dilution of 1:100 - 1:1000. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:50 - 1:500. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., chromatin immunoprecipitation, immunohistochemistry, immunoprecipitation, Western Blot
Background This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.
General Readings
  1. Mowen KA, Tang J, Zhu W, Schurter BT, Shuai K, Herschman HR, et al. Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription. Cell. 2001 Mar 9;104(5):731-41. PubMed PMID: 11257227.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera
Buffer System:
No Preservative
Whole antisera
This reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the reacts with symmetric dimethyl arginine.
All Species

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