AP02330PU-N ATF2 pSer62/44 antibody

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0.1 mg / €300.00
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Quick Overview

Rabbit anti Human, Mouse ATF2

Product Description for ATF2

Rabbit anti Human, Mouse ATF2.
Properties: pSer62/44
Presentation: Aff - Purified
Product is tested for Paraffin Sections.

Properties for ATF2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2
Presentation Aff - Purified
Reactivity Hu, Ms
Applications P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P15336
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ATF-2 around the phosphorylation site of serine 62 or 44 (N-D-SP-V-I).
GeneID:
1386
Property pSer62/44
Application Suitable for use in Immunohistochemistry (1:50~1:100).
Background ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.
Concentration 1.0 mg/ml
General Readings
  1. Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.
  2. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181(2): 629-635.
  3. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393.
  4. Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Immunoaffinity chromatography.
Buffer System:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% glycerol.
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
ATF-2 (phospho-Ser62 or 44) antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 62 or 44.
Species:
Human, Mouse and Rat.
Gene ID 1386
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for ATF2 (16 products)

Catalog No. Species Pres. Purity   Source  

ATF2 (full length, C-term polyhistidine tag)

ATF2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

ATF2 (full length, N-term HIS tag)

ATF2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €205.00
  OriGene Technologies, Inc.

ATF2

ATF2 Human Purified >90.0% as determined by SDS-PAGE E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human Purified >90.0% as determined by SDS-PAGE E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human Purified >90.0% as determined by SDS-PAGE E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human Purified > 95 % Greater than 90% as determined by SDS-PAGE. E. coli
  OriGene Technologies GmbH

ATF2

ATF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ATF2

ATF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ATF2

ATF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ATF2

ATF2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

ATF2

ATF2 Human Purified
  Abnova Taiwan Corp.

ATF2

ATF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ATF2

ATF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

ATF2

ATF2 Human
  Abnova Taiwan Corp.

Positive controls for ATF2 (1 products)

Catalog No. Species Pres. Purity   Source  

ATF2 Lysate(Denatured)

ATF2 Lysate(Denatured)
  Abnova Taiwan Corp.
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