NB600-918 ATPase (Na/K) antibody

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Chicken anti Canine ATPase (Na/K)


Product Description for ATPase (Na/K)

Chicken anti Canine ATPase (Na/K).
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for ATPase (Na/K)

Product Category Primary Antibodies
Quantity 1 mg
Synonyms ATPase Sodium/Potasium
Presentation Aff - Purified
Reactivity Can
Applications E, IP, WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

ATPase [sodium, potassium] from canine kidney.
Add. information Research Areas: Cellular Markers, Neuroscience
Application ELISA, Immunocytochemistry, immunofluorescence, Immunohistochemistry-Frozen, Western Blot, This antibody can be used for ELISA, ICC, IF, IHC-Fr, WB. Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-chicken serum, as well as purified and partially purified ATPase (sodium, potassium) from canine kidney. ELISA: This product has been assayed against 1.0 ug of ATPase (sodium, potassium) in a standard sandwich ELISA using peroxidase-conjugated, affinity purified rabbit anti-Chicken IgG [H&L] and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. ICC/IF: Use amplification., ELISA 1:1000-1:6000, immunofluorescence 1:500, Western Blot 1:100 - 1:500, Immunocytochemistry 1:500, Immunohistochemistry-Frozen 1:100
Background Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase a1 subunit, in brain, is expressed in both neuronal and non-neuronal cells.
Concentration 10 mg/ml
General Readings
  1. Lee J, Shirk A, Oram JF, Lee SP, Kuver R. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J. 2002 Jun 1;364(Pt 2):475-84. PubMed PMID: 12023891. (Free PMC Article available)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
Sodium Azide
Immunogen affinity purified
Aff - Purified
ATPase (Na/K) - Plasma Membrane Marker
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