AM09372PU-N Aurora kinase B antibody

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0.1 ml / €310.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Aurora kinase B AT2B1

Product Description for Aurora kinase B

Mouse anti Human Aurora kinase B AT2B1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Aurora kinase B

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12
Presentation Purified
Reactivity Hu
Applications E, F, ICC/IF, WB
Clonality Monoclonal
Clone AT2B1
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q96GD4
Immunogen:
Recombinant human Aurora kinase B (1-344aa) purified from E. coli
GeneID:
9212
Isotype control AM03110PU-N, SM12P
Application ELISA. 
WesternBlot.

Flow Cytometry.
Immunofluorescence: 1/1000-1/2000.
Background The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells.
Concentration 1.0 mg/ml
General Readings
  1. Posch M, Khoudoli GA, Swift S, King EM, Deluca JG, Swedlow JR. Sds22 regulates aurora B activity and microtubule-kinetochore interactions at mitosis. J Cell Biol. 2010 Oct 4;191(1):61-74. doi: 10.1083/jcb.200912046. PubMed PMID: 20921135. (Free PMC Article available, 7 images available)
  2. Hall LL, Byron M, Pageau G, Lawrence JB. AURKB-mediated effects on chromatin regulate binding versus release of XIST RNA to the inactive chromosome. J Cell Biol. 2009 Aug 24;186(4):491-507. doi: 10.1083/jcb.200811143. PubMed PMID: 19704020. (Free PMC Article available, 10 images available)
  3. Smith SL, Bowers NL, Betticher DC, Gautschi O, Ratschiller D, Hoban PR, et al. Overexpression of aurora B kinase (AURKB) in primary non-small cell lung carcinoma is frequent, generally driven from one allele, and correlates with the level of genetic instability. Br J Cancer. 2005 Sep 19;93(6):719-29. PubMed PMID: 16222316. (Free PMC Article available, 4 images available)
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State:
Liquid purified Ig fraction
Purified
Specificity
Specificity:
The antibody recognizes Human Aurora kinase B. Other species not tested.
Gene ID 9212
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for Aurora kinase B (12 products)

Catalog No. Species Pres. Purity   Source  

Aurora kinase B

Aurora kinase B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Aurora kinase B (1-344, His-tag)

Aurora kinase B Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €1,500.00
  OriGene Technologies GmbH

Aurora kinase B (1-344, His-tag)

Aurora kinase B Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €520.00
  OriGene Technologies GmbH

Aurora kinase B

Aurora kinase B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human
  Abnova Taiwan Corp.

Aurora kinase B

Aurora kinase B Human
  Abnova Taiwan Corp.

Aurora kinase B

SDS-Page: Aurora B Protein [NBP1-30242] - Aurora Kinase B, 41.4 kDa (364aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE
  Novus Biologicals Inc.

Positive controls for Aurora kinase B (2 products)

Catalog No. Species Pres. Purity   Source  

AURKB overexpression lysate

AURKB overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Aurora B Lysate

Western Blot: Aurora B Lysate [NBL1-07860] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for AURKB Protein
  Novus Biologicals Inc.
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