AM08110BT-N B-Cells (subset) antibody

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0.5 mg / €480.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Porcine B-Cells BB6-10A10

AM08110BT-N

Product Description for B-Cells

Mouse anti Porcine B-Cells BB6-10A10.
Properties: (subset)
Presentation: Biotin
Product is tested for Flow Cytometry.

Properties for B-Cells

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms B cells, B-cell marker
Presentation Biotin
Reactivity Por
Applications F
Clonality Monoclonal
Clone BB6-10A10
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Property subset
Application Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1-2)
Background B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.
Concentration 0.5 mg/ml
General Readings
  1. Denham S, Zwart RJ, Whittall JT, Pampusch M, Corteyn AH, Bianchi AT, et al. Monoclonal antibodies putatively identifying porcine B cells. Vet Immunol Immunopathol. 1998 Jan 30;60(3-4):317-28. PubMed PMID: 9589570.
  2. Pescovitz, M. Personal communication.
Storage Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Buffer System:
PBS containing 0.09% Sodium Azide as preservative.
State:
Liquid purified Ig fraction.
Specificity
Specificity:
This antibody stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus (Ref.1).
While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.
Species:
Pig.

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