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Mouse anti Human B-Cells MB3


Product Description for B-Cells

Mouse anti Human B-Cells MB3.
Presentation: Liquid
Product is tested for Western blot / Immunoblot, Frozen Sections, Paraffin Sections.

Properties for B-Cells

Product Category Primary Antibodies
Quantity 1 ml
Presentation Liquid
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone MB3
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isolated from Human lymphocytes.
Application Immunoblotting.
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections.
Recommended Dilutions: 1/5-1/20.
Positive Control: Lymph nodes, Tonsil. 
Incubation time: 60 min at RT.
Pretreatment: No protease pre-treatment required, blocking of endogenous peroxidases is recommended.
General Readings
  1. Poppema S, Hollema H, Visser L, Vos H. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol. 1987 Jun;127(3):418-29. PubMed PMID: 3296769. (Free PMC Article available)
  2. Fukuda T, Ohnishi Y, Hasegawa T, Kakihara T, Usuda H. Distribution of antigens detected with MB1, MB2 and MB3 on non-hematopoietic human organs and various tumors. Acta Pathol Jpn. 1992 May;42(5):339-46. PubMed PMID: 1636436.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
Stabilizing phosphate buffer, pH 7.3
0.09% Sodium Azide
Liquid Concentrate
Species Reactivity
Species reactivity (tested):
The antibody MB3 is an excellent marker of all B cells. It is especially well suited for the immunocytochemical and histochemical detection of B cells in formaldehyde fixed tissue (also Bouin's fixative).
It is used for the immunophenotyping of lymphoma and multiple myeloma. It does not react with normal T cells but only with activated ones.
In non-haematopoietic tissue MB3 especially reacts with histiocytes, glandular epithelia, smooth muscle, endothelial cells and peripheral nerve tissue. 
MB3 recognizes a 31 kDa protein fraction on human B-lymphocytes.

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