BM5116S B-Cells antibody

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1 ml / €400.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human B-Cells MB2


Product Description for B-Cells

Mouse anti Human B-Cells MB2.
Presentation: Supernatant
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for B-Cells

Product Category Primary Antibodies
Quantity 1 ml
Presentation Supernatant
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone MB2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isolated from Human lymphocytes
Application Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections:
For use in paraffin embedded tissue no antigen preteatment is needed but better results could be obtained if a TUF pretreatment is preformed with an antibody dilution of 1/20.
Recommended Dilutions: Working dilution for regular immunohistochemistry is approximately 1/5-1/40, but the optimal dilution should be tested by serial dilutions.
Recommended Positive Control: Tonsil, Lymph nodes. 
Incubation time: 60 min at RT.
General Readings
  1. Poppema S, Hollema H, Visser L, Vos H. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol. 1987 Jun;127(3):418-29. PubMed PMID: 3296769. (Free PMC Article available)
  2. Hall PA, D'Ardenne AJ, Butler MG, Habeshaw JR, Stansfeld AG. New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections. J Clin Pathol. 1987 Feb;40(2):151-6. PubMed PMID: 3546393. (Free PMC Article available)
  3. Dehou MF, Schots R, Lacor P, Arras N, Verhavert P, Klöppel G, et al. Diagnostic and prognostic value of the MB2 monoclonal antibody in paraffin-embedded bone marrow sections of patients with multiple myeloma and monoclonal gammopathy of undetermined significance. Am J Clin Pathol. 1990 Sep;94(3):287-91. PubMed PMID: 1697732.
  4. Schmid C, Pan L, Diss T, Isaacson PG. Expression of B-cell antigens by Hodgkin's and Reed-Sternberg cells. Am J Pathol. 1991 Oct;139(4):701-7. PubMed PMID: 1656757. (Free PMC Article available)
  5. Fukuda T, Ohnishi Y, Hasegawa T, Kakihara T, Usuda H. Distribution of antigens detected with MB1, MB2 and MB3 on non-hematopoietic human organs and various tumors. Acta Pathol Jpn. 1992 May;42(5):339-46. PubMed PMID: 1636436.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0.09% Sodium Azide
Liquid Culture Supernatant
Species Reactivity
Species reactivity (tested):
The antibody MB2 is an excellent Marker for lymphoma diagnosis. This MB2 antibody  reacts with all B-cells. Further positive reaction with epithelia and endothelium was observed.
MB2 recognizes neuraminidase-resistant cytoplasmic 28 kDa antigen, expressed strongly on B-cells and weakly on mature T-cells; no reaction with immature T-cells; negative staining with plasmacytomas.

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