NB100-56153 Baculovirus p35 antibody

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50 µl / €340.00
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Quick Overview

Rabbit anti Baculovirus p35

Product Description for Baculovirus p35

Rabbit anti Baculovirus p35.
Presentation: Serum
Product is tested for Frozen Sections, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Baculovirus p35

Product Category Primary Antibodies
Quantity 50 µl
Presentation Serum
Applications C, IP, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P31354
Immunogen:
Full-length recombinant p35 baculovirus protein was used as immunogen.
GeneID:
1488744
Application Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, This antibody is useful for immunoprecipitation, Western Blot, Immunohistochemistry-Paraffin and Immunohistochemistry-Frozen ., immunoprecipitation , Western Blot 1/1000 - 1/2000, Immunohistochemistry-Paraffin 1/1000 - 1/5000, Immunohistochemistry-Frozen Use at an assay dependent dilution
Background The baculoviruses are a large, diverse family of DNA viruses that have evolved a number of mechanisms to manipulate thier insect hosts. One of these is the ability to regulate apoptosis during infection by expressing proteins that can inhibit caspase activation, including the caspase inhibitor p35 and the inhibitor of apoptosis (IAP) proteins (reviewed in Clem, 2005; Clarke and Clem, 2003; and Iller, 1997). The p35 baculovirus protein strongly inhibits caspase enzymatic activity, and is the the most broadly acting caspase inhibitor protein known. p35 baculovirus forms essentially irreversible complexes with its target caspases in a process that is accompanied by the cleavage of p35, generating two fragments of approximately 10 kDa and 25 kDa. These cleavage fragments remain associated with caspases and thereby block caspase activity. The ability of p35 to inhibit caspases along with the central role of caspases in the apoptotic process enables p35 baculovirus to block apoptosis in a phylogentically broad range of cells, and in response to a wide variety of apoptotic induction signals. For example, over expression of p35 in mammalian, insect, and nematode cells results in resistance to apoptosis. IMG-5740 recognizes p35 baculovirus; p35 baculovirus migrates at ~35 kDa on SDS-PAGE.
General Readings
  1. Clem RJ. The role of apoptosis in defense against baculovirus infection in insects. Curr Top Microbiol Immunol. 2005;289:113-29. PubMed PMID: 15791953.
  2. Clarke TE, Clem RJ. Insect defenses against virus infection: the role of apoptosis. Int Rev Immunol. 2003 Sep-Dec;22(5-6):401-24. PubMed PMID: 12959752.
  3. Miller LK. Baculovirus interaction with host apoptotic pathways. J Cell Physiol. 1997 Nov;173(2):178-82. PubMed PMID: 9365518.
  4. Riedl SJ, Renatus M, Snipas SJ, Salvesen GS. Mechanism-based inactivation of caspases by the apoptotic suppressor p35. Biochemistry. 2001 Nov 6;40(44):13274-80. PubMed PMID: 11683637.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Whole antisera
Buffer System:
0.05% Sodium Azide
Purity:
Whole antisera
State:
Serum
Specificity
Specificity:
p35 baculovirus may be observed as one or multiple bands on western blot: p35 baculovirus is cleaved during its reaction with caspases. This results in cleavage of p35; 10 and 25 kDa fragments have been described. Additional breakdown p35 products may also result.
Species:
Multiple specie cross reactivity. Not yet tested in other species.
Gene ID 1488744

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