AM26364PU-N Barmotin antibody

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Mouse anti Canine, Chicken, Human, Mouse, Rat Barmotin 7H6


Product Description for Barmotin

Mouse anti Canine, Chicken, Human, Mouse, Rat Barmotin 7H6.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Frozen Sections.

Properties for Barmotin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Can, Chk, Hu, Ms, Rt
Applications C, WB
Clonality Monoclonal
Clone 7H6
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control SM13P
Application Immunohistochemistry on frozen sections: The typical starting working dilution is 1:10.
Western blot: The typical starting working dilution is 1:10.

Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. The monoclonal antibody 7H6 reacts with a 155 kD tight junction-associated protein also designated barmotin. This protein is preferentially localized at the periphery of tight junctions of hepatocytes and other epithelia. The 7H6 antigen is also detectable in the intestine and kidney. Studies with Madin-Darby canine kidney (MDCK) cells indicate the 7H6 antigen to be an important molecule for regulation of the barrier function of tight junctions.
Confocal laser scanning microscopy revealed that the expression of 7H6 antigen decreased sequentially during hepatocarcinogenesis. The 7H6 antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern.
Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of 7H6 expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor.

Concentration 0.1 mg/ml
General Readings
  1. Zhong Y, Saitoh T, Minase T, Sawada N, Enomoto K, Mori M. Monoclonal antibody 7H6 reacts with a novel tight junction-associated protein distinct from ZO-1, cingulin and ZO-2. J Cell Biol. 1993 Jan;120(2):477-83. PubMed PMID: 8421059. (Free PMC Article available)
  2. Kojima T, Sawada N, Zhong Y, Oyamada M, Mori M. Sequential changes in intercellular junctions between hepatocytes during the course of acute liver injury and restoration after thioacetamide treatment. Virchows Arch. 1994;425(4):407-12. PubMed PMID: 7820303.
  3. Zhong Y, Enomoto K, Tobioka H, Konishi Y, Satoh M, Mori M. Sequential decrease in tight junctions as revealed by 7H6 tight junction-associated protein during rat hepatocarcinogenesis. Jpn J Cancer Res. 1994 Apr;85(4):351-6. PubMed PMID: 8200847.
  4. Zhong Y, Enomoto K, Isomura H, Sawada N, Minase T, Oyamada M, et al. Localization of the 7H6 antigen at tight junctions correlates with the paracellular barrier function of MDCK cells. Exp Cell Res. 1994 Oct;214(2):614-20. PubMed PMID: 7925655.
  5. Kimura M, Sawada N, Kimura H, Isomura H, Hirata K, Mori M. Comparison between the distribution of 7H6 tight junction-associated antigen and occludin during the development of chick intestine. Cell Struct Funct. 1996 Feb;21(1):91-6. PubMed PMID: 8726478.
  6. Kimura H, Sawada N, Tobioka H, Isomura H, Kokai Y, Hirata K, et al. Bacterial lipopolysaccharide reduced intestinal barrier function and altered localization of 7H6 antigen in IEC-6 rat intestinal crypt cells. J Cell Physiol. 1997 Jun;171(3):284-90. PubMed PMID: 9180897.
  7. Muto S, Sato Y, Umeki Y, Yoshida K, Yoshioka T, Nishikawa Y, et al. HGF/SF-induced spreading of MDCK cells correlates with disappearance of barmotin/7H6, a tight junction-associated protein, from the cell membrane. Cell Biol Int. 2000;24(7):439-46. PubMed PMID: 10875891.
Storage Store at 2 - 8 °C.
Shelf life: one year from despatch.
Protein G
Buffer System:
0.02% sodium azide
0.2% bovine serum albumin
Liquid Ig fraction
Species Reactivity
Species reactivity (tested):
Human, canine, mouse, rat, chicken
This antibody detects Barmotin.

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