AM26133PU-N Basal Membrane antibody

See related secondary antibodies

Search for all "Basal Membrane"

0.2 ml / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Porcine Basal Membrane PB1

AM26133PU-N

More Views

  • AM26133PU-N
  • AM26133PU-N

Product Description for Basal Membrane

Mouse anti Human, Porcine Basal Membrane PB1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Paraffin Sections.

Properties for Basal Membrane

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms Epithelial Basement cells
Presentation Purified
Reactivity Hu, Por
Applications C, E, P
Clonality Monoclonal
Clone PB1
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Porcine lung extract
Isotype control SM13P
Add. information Antigen Distribution on Tissue sections: Basal cell layer of various epithelia in different swine organs, e.g. basal cells in the epidermis of the skin, in bronchiolar epithelia of the lung and in cells of the macula densa in the kidney.
Application ELISA.
Immunohistochemistry on Frozen Sections: 1/200.
Immunohistochemistry on Paraffin Sections: 1/30 (Proteinase K pretreatment for antigen retrieval is recommended).

Concentration 1.0 mg/ml
Protocols Staining Procedure For Frozen And Paraffin-EmbeddedTissue Sections 
Protocol with frozen, ice-cold acetone-fixed sections:
The whole procedure is performed at room temperature
1. Wash in PBS
2. Block endogenous peroxidase
3. Wash in PBS
4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
6. Wash in PBS
7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse lgM) for 1h in a humid chamber
8. Wash in PBS
9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
10. Wash in PBS
11. Counterstain with Mayer’s hemalum. 

Protocol with formalin-fixed, paraffin-embedded sections:
The whole procedure is performed at room temperature
1. Deparaffinize and rehydrate tissue section
2. Incubate the tissue section with proteinase K for 7min
3. Block endogenous peroxidase
4. Wash in PBS
5. Block with 10% normal goat serum in PBS for 30min. in a humid chamber
6. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber
7. Wash in PBS
8. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgM) for 1h in a humid chamber
9. Wash in PBS
10. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.
11. Wash in PBS
12. Counterstain with Mayer’s hemalum
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
PBS, pH 7.2
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Swine.
Specificity
Specificity:
This antibody recognizes cells of the epithelial basement cell layer in various tissues.

Accessory Products

  • LinkedIn