AM10030FC-S basic Cytokeratin antibody

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0.1 mg / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat basic Cytokeratin AE3


Product Description for basic Cytokeratin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat basic Cytokeratin AE3.
Presentation: FITC
Product is tested for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Properties for basic Cytokeratin

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Bov, Chk, Hu, Mky, Ms, Por, Rb, Rt
Applications F, ICC/IF
Clonality Monoclonal
Clone AE3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human epidermal keratin.
Molecular Weight of Antigen: CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5).
Isotype control AM03095PU-N, SM10F (for use in human samples), SM20F (for use in rat samples)
Application Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC.
Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Concentration 0.2 mg/ml
General Readings
  1. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11-24. PubMed PMID: 6186379.
  2. Sun TT, Tseng SC, Huang AJ, Cooper D, Schermer A, Lynch MH, et al. Monoclonal antibody studies of mammalian epithelial keratins: a review. Ann N Y Acad Sci. 1985;455:307-29. PubMed PMID: 2417518.
  3. Tseng SC, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TT. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell. 1982 Sep;30(2):361-72. PubMed PMID: 6183000.
  4. Kuraguchi M, Wang XP, Bronson RT, Rothenberg R, Ohene-Baah NY, Lund JJ, et al. Adenomatous polyposis coli (APC) is required for normal development of skin and thymus. PLoS Genet. 2006 Sep 15;2(9):e146. Epub 2006 Jul 28. PubMed PMID: 17002498. (Free PMC Article available, 6 images available)
  5. Battifora H, Sheibani K, Tubbs RR, Kopinski MI, Sun TT. Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma. Cancer. 1984 Sep 1;54(5):843-8. PubMed PMID: 6204739.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. 
This product is photosensitive and should be protected from light. 
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Protein A Chromatography
Buffer System:
PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative
Liquid purified Ig fraction
This antibody reacts with CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58) CK6(56) and CK8 (52.5).
Cellular Localization:Cytoplasmic.
Human, Monkey, Bovine, Porcine, Rat, Mouse, Rabbit and Chicken.

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