AM33272PU-T basic Cytokeratin antibody

See related secondary antibodies

Search for all "basic Cytokeratin"

0.1 mg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat basic Cytokeratin SPM116

Product Description for basic Cytokeratin

Mouse anti Bovine, Canine, Chicken, Human, Monkey, Mouse, Rabbit, Rat basic Cytokeratin SPM116.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for basic Cytokeratin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cytokeratin type II, HMW Cytokeratins, Keratin type I
Presentation Purified
Reactivity Bov, Can, Chk, Hu, Mky, Ms, Rb, Rt
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone SPM116
Host Mouse
Isotype IgG1
Molecular weight 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human epidermal keratin.
Isotype control SM20P (for use in rat samples)
Application ELISA: Use BSA free Antibody for Coating.
Western Blot:
0.5-1 µg/ml.
Flow Cytometry: 0.5-1 µg/106 cells.
Immunofluorescence: 1-2 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 0.5-1 µg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: Skin, Adenocarcinomas.
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Concentration 0.2 mg/ml
General Readings
  1. Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8. PubMed PMID: 6183275. (Free PMC Article available)
  2. Tseng SCG et. al. Cell 1982; 30361.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor concentrate
Purified
Species Reactivity
Species reactivity (tested):
Human, Monkey, Cow, Dog, Rabbit, Mouse, Rat, Chicken.
Specificity
Specificity:
This Monoclonal antibody (Clone SPM116) recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI < 5.7) and basic (pI > 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa.
This SPM116 antibody recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Cellular Localization: Cytoplasmic.

Accessory Products

  • LinkedIn