DDX0030P-100 Basophils antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Basophils 212H6.16


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Product Description for Basophils

Mouse anti Human Basophils 212H6.16.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Western blot / Immunoblot.

Properties for Basophils

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Basophil cells
Presentation Purified
Reactivity Hu
Applications C, F, WB
Clonality Monoclonal
Clone 212H6.16
Host Mouse
Isotype IgM
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Enriched-human tonsillar pDCs
Isotype control SM13P
Application Cell sorting, ImmunoHistoChemistry frozen sections, Western Blot. 
Applications tested:
MACS- separation and flow cytometry, IHC frozen section. 
Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
Background Basophils and mast cells are multifunctional hematopoietic effector cells involved in allergic and inflammatory reactions. Immediate allergy is caused by release of chemical mediators by these cell types. In human peripheral blood, 212H6.16 is an antibody specific to a yet non-identified cell-surface structure present on basophils. 212H6.16 does not induce basophil degranulation, and is therefore a valuable tool for the isolation and study of human basophils. Tonsillar epithelium and skin keratinocytes are also stained by 212H6 in situ: antigen expression in tonsil is restricted to cells lining the epithelial crypts.
(Bendriss-Vermare N.et al, Leukocyte Typing VII, White Cell Differentiation Antigen MC13, part 9, myeloid cells, 425-30, 2001; Editor Mason).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Arock M, Schneider E, Boissan M, Tricottet V, Dy M. Differentiation of human basophils: an overview of recent advances and pending questions. J Leukoc Biol. 2002 Apr;71(4):557-64. PubMed PMID: 11927641.
  2. Salvatore Chirumbolo. State-of-the-art review about basophil research in immunology and allergy: is the time right to treat these cells with the respect they deserve?. Blood Transfus 2012; 10: 148-64. DOI 10.2450/2011.0020-11.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
QMA Hyper D Ion Exchange Chromatography
100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
Human basophils and mast cells. 
Species cross-reactivity: no-cross reactivity on Mouse Basophil.

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