discontinued

AM33088PU-N Bcl-11B antibody

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Rat anti Human, Mouse, Zebrafish Bcl-11B 25B6

AM33088PU-N

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  • AM33088PU-N

Product Description for Bcl-11B

Rat anti Human, Mouse, Zebrafish Bcl-11B 25B6.
Presentation: Purified
Product is tested for ChIP assay, Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Bcl-11B

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B, BCL11B, COUP-TF-interacting protein 2, CTIP2, RIT1, Radiation-induced tumor suppressor gene 1 protein
Presentation Purified
Reactivity Hu, Ms, Ze
Applications ChIP, ICC/IF, P, WB
Clonality Monoclonal
Clone 25B6
Host Rat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9C0K0
Immunogen:
Human Bcl11b.
GeneID:
64919
Application Western blot: Each lot of this antibody is quality control tested. 
Use 0.5 µg/ml antibody dilution buffer for each mini-gel. 
Immunofluorescence: Use a dilution of 1/200. 
CHIP. 
Immunohistochemistry. 
See also Application References 1-3.
Background The transcription factor Bcl11b is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies. 
Structure: 894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution: Nucleus.
Function: Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction: Interacts with TFCOUP1, SIRT1, ARP1, and EAR2.
Concentration 0.5 mg/ml
General Readings
  1. Tomassy GS, De Leonibus E, Jabaudon D, Lodato S, Alfano C, Mele A, et al. Area-specific temporal control of corticospinal motor neuron differentiation by COUP-TFI. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3576-81. doi: 10.1073/pnas.0911792107. Epub 2010 Feb 2. PubMed PMID: 20133588. (Free PMC Article available, 6 images available)
  2. Cherrier T, Suzanne S, Redel L, Calao M, Marban C, Samah B, et al. p21(WAF1) gene promoter is epigenetically silenced by CTIP2 and SUV39H1. Oncogene. 2009 Sep 24;28(38):3380-9. doi: 10.1038/onc.2009.193. Epub 2009 Jul 6. PubMed PMID: 19581932. (Free PMC Article available, 6 images available)
  3. Kosulin K, et al. 2013. PLoS One. 8:63646.
  4. Liu P, et al. 2011. Immunol. Rev. 238:138.
  5. Ikawa T, Hirose S, Masuda K, Kakugawa K, Satoh R, Shibano-Satoh A, et al. An essential developmental checkpoint for production of the T cell lineage. Science. 2010 Jul 2;329(5987):93-6. doi: 10.1126/science.1188995. PubMed PMID: 20595615.
  6. Li L, Leid M, Rothenberg EV. An early T cell lineage commitment checkpoint dependent on the transcription factor Bcl11b. Science. 2010 Jul 2;329(5987):89-93. doi: 10.1126/science.1188989. PubMed PMID: 20595614. (Free PMC Article available, 3 images available)
  7. Li P, Burke S, Wang J, Chen X, Ortiz M, Lee SC, et al. Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion. Science. 2010 Jul 2;329(5987):85-9. doi: 10.1126/science.1188063. Epub 2010 Jun 10. PubMed PMID: 20538915. (Free PMC Article available, 4 images available)
  8. Tydell CC, David-Fung ES, Moore JE, Rowen L, Taghon T, Rothenberg EV. Molecular dissection of prethymic progenitor entry into the T lymphocyte developmental pathway. J Immunol. 2007 Jul 1;179(1):421-38. PubMed PMID: 17579063.
  9. Avram D, Fields A, Senawong T, Topark-Ngarm A, Leid M. COUP-TF (chicken ovalbumin upstream promoter transcription factor)-interacting protein 1 (CTIP1) is a sequence-specific DNA binding protein. Biochem J. 2002 Dec 1;368(Pt 2):555-63. PubMed PMID: 12196208. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
Hepes-buffered solution, pH 7.5
Preservatives:
0.02% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This 25B6 monoclonal antibody recognizes Human, Mouse and Zebrafish Bcl-11B.

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