AM00008PU-N Bcl-2-like 8 antibody

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0.1 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Bcl-2-like 8 5E6

AM00008PU-N

Product Description for Bcl-2-like 8

Mouse anti Human Bcl-2-like 8 5E6.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Bcl-2-like 8

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8
Presentation Purified
Reactivity Hu
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone 5E6
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q92934
Immunogen:
Recombinant Human BAD.
GeneID:
572
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Molecular Weight. 19 kDa
Application Immunoprecipitation: 1-2 µg/ml.
Also suitable for Western blotting if cells overexpressing BAD are used (0.5 µg/ml for HRPO/ECL detection).
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Immunocytochemistry. 
Note
We recommend to use mab BAD-5E6 for IP applications. The mab is also suitable for Western-blot applications if cells overexpressing BAD are used.
Background BAD is an important regulatory protein controlling cellsurvival/apoptosis. Unphosphorylated BAD heterodimerizes with Bcl-2 and Bcl-XL, thus neutralizing the anti-apoptotic properties of the Bcl proteins. Activation of different signal transduction pathways leads to phosphorylation of BAD at multiple serine residues, e.g. phosphorylation of serine 136 by PKB, serine 112 by MAPK, and serine 155 by PKA, and dissociation of the BAD/Bcl heterodimers.
Storage For long-term storage, freeze lyophilizate upon arrival (-20°C).
Upon reconstitution, aliquote and freeze in liquid nitrogen.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Subsequent Thiopilic Adsorption and Size Exclusion Chromatography
Buffer System:
1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose
State:
Lyophilized purified IgG fraction.
Purified
Reconstitution:
Restore with 1 ml H2O (15 min, RT).
Specificity
Specificity:
This antibody specifically binds the BAD protein but does not recognize BAD/Bcl-2 heterodimers in Immunoprecipitation assays.
Species:
Human.
Gene ID 572
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Bcl-2-like 8 (15 products)

Catalog No. Species Pres. Purity   Source  

Bcl-2-like 8 (transcript variant 1)

Bcl-2-like 8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Bcl-2-like 8 (full length, N-term HIS tag, transcript variant 2)

Bcl-2-like 8 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Positive controls for Bcl-2-like 8 (1 products)

Catalog No. Species Pres. Purity   Source  

Bad Lysate

Western Blot: Bad Lysate [NBL1-07900] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for BAD. Protein
  Novus Biologicals Inc.
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