AP02799PU-N Bcl-2-like 8 pSer136/99 antibody

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0.1 ml / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat Bcl-2-like 8

Product Description for Bcl-2-like 8

Rabbit anti Human, Mouse, Rat Bcl-2-like 8.
Properties: pSer136/99
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for Bcl-2-like 8

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q92934
Immunogen:
Synthesized phosphopeptide derived from mouse BAD around the phosphorylation site of serine136
AA Sequence:
S-R-SP-A-P
GeneID:
572
Property pSer136/99
Application Immunohistochemistry on paraffin sections: 1:50 - 1:100.
Western blot: see ref. 1.
Background Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Concentration 1.0 mg/ml
General Readings
  1. Matsuda N, Takano Y, Kageyama S, Hatakeyama N, Shakunaga K, Kitajima I, et al. Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Cardiovasc Res. 2007 Oct 1;76(1):132-40. Epub 2007 Jun 5. PubMed PMID: 17601517.
Storage Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity chromatography using epitope-specific phosphopeptide
Buffer System:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Specificity
Specificity:
BAD (phospho-Ser136) antibody detects endogenous levels of BAD only when phosphorylated at Serine 136.
Species:
Human, Mouse, Rat.
Gene ID 572
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Bcl-2-like 8 (15 products)

Catalog No. Species Pres. Purity   Source  

Bcl-2-like 8 (transcript variant 1)

Bcl-2-like 8 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Bcl-2-like 8 (full length, N-term HIS tag, transcript variant 2)

Bcl-2-like 8 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human Purified E. coli
  Acris Antibodies GmbH

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Bcl-2-like 8

Bcl-2-like 8 Human
  Abnova Taiwan Corp.

Positive controls for Bcl-2-like 8 (1 products)

Catalog No. Species Pres. Purity   Source  

Bad Lysate

Western Blot: Bad Lysate [NBL1-07900] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for BAD. Protein
  Novus Biologicals Inc.
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