discontinued

NBP1-22572 Bcl2 (100/D5) antibody

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0.5 ml / €360.00

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Mouse anti Human Bcl2 (100/D5) 100/D5

Product Description for Bcl2 (100/D5)

Mouse anti Human Bcl2 (100/D5) 100/D5.
Presentation: Purified
Product is tested for Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for Bcl2 (100/D5)

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms anti-Apoptosis regulator Bcl 2 antibody, anti-Apoptosis regulator Bcl2 antibody, anti-B cell CLL/lymphoma 2 antibody, anti-B cell leukemia/lymphoma 2 antibody, anti-B cell lymphoma 2 antibody, anti-BCL2 protein antibody, anti-Bcl 2 antibody, anti-Bcl2 antibody
Presentation Purified
Reactivity Hu
Applications IP, P, WB
Clonality Monoclonal
Clone 100/D5
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
BALB/C mice were injected with synthetic bcl-2 peptide.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application immunohistochemistry, immunoprecipitation, Western Blot, Immunoprecipitation, Western Blot and ABC, immunoprecipitation 1:50, Western Blot 1:50-1:100, immunohistochemistry 1:25-1:50
Background BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL.
Storage Store at 4C. Do not freeze.
Format
Purification:
IgG purified
Buffer System:
Sodium Azide
Purity:
IgG purified
State:
Purified
Specificity
Specificity:
The bcl-2 product is considered to act as an inhibitor of apoptosis. bcl-2 expression is inhibited in germinal centers where apoptosis forms part of B cell production pathway. Reactive follicles show no staining for bcl-2, where as the cells in neoplastic follicles exhibit membrane staining.
Species:
Human

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