AP02468PU-N BCR pTyr177 antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse BCR

AP02468PU-N

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  • AP02468PU-N

Product Description for BCR

Rabbit anti Human, Mouse BCR.
Properties: pTyr177
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for BCR

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BCR1, Breakpoint cluster region protein, D22S11, NY-REN-26, Renal carcinoma antigen NY-REN-26
Presentation Aff - Purified
Reactivity Hu, Ms
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P11274
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from Human Bcr around the phosphorylation site of Tyrosine 177 (P-F-Yp-V-N).
GeneID:
613
Property pTyr177
Add. information Molecular Weight: 210 kDa
Application Suitable for use in Western blot (1/500-1/1000) and Immunohistochemistry on Paraffin-Embedded Sections (1/50-1/100).
Background The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Concentration 1.0 mg/ml
General Readings
  1. Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
  2. Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
  3. Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
  4. Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907–30916.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol
State:
Liquid purified Ig fraction.
Aff - Purified
Specificity
Specificity:
This antibody detects endogenous levels of Bcr only when phosphorylated at Tyrosine 177.
Species:
Human and Mouse.
Gene ID 613

Accessory Products

Proteins and/or Positive Controls

Proteins for BCR (3 products)

Catalog No. Species Pres. Purity   Source  

BCR (transcript variant 2)

BCR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

BCR

BCR Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

BCR

BCR Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for BCR (2 products)

Catalog No. Species Pres. Purity   Source  

BCR 293T Cell Transient Overexpression Lysate(Denatured)

BCR 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

BCR overexpression lysate

BCR overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
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