discontinued

AP09915PU-N Bestrophin-4 (C-term amidated) antibody

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Rabbit anti Mouse, Rat Bestrophin-4

Product Description for Bestrophin-4

Rabbit anti Mouse, Rat Bestrophin-4.
Properties: (C-term amidated)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Bestrophin-4

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms BEST4, VMD2L2, Vitelliform macular dystrophy 2-like protein 2
Presentation Aff - Purified
Reactivity Ms, Rt
Applications E, IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8NFU0
Immunogen:
Synthetic peptide for Bestrophin 4 close to C-terminal corresponding to amino acids 457-473
AA Sequence:
ari eee sae sgd eal ep
GeneID:
266675
Remarks:
The peptide was post-synthetically modified to achieve
desired immunogenecity. The carboxy peptide were amidated before conjugation.
Property C-term amidated
Application ELISA.
Western blot > 1:500.
Immunoprecipitation and i.p pull-down assays: > 1:200.
Background In the recent past at least 3 different family for “chloride channel” have been characterized: ClC family, ligand gated channels for the GABA and glycine receptor family and the cystic fibrosis membrane conductance regulator. These chloride channels play important role in mainting resting potentials, ion refluxes, acidification of internal organnles such as lysosomes in both excitatory and non-excitatory nerve and muscle cells. The bestrophins are a newly described family of “anion channels” unrelated in primary sequence to any previously characterized channel proteins. Bestrophins were originally defined as a family of over 20 related sequences of the C. elegans. The first mammalian bestrophin was identified as the vitelliform macular dystrophy (VMD), 1 also known as Best disease (1). Three more members of the bestrophin family members were cloned andindenfied recently, Bestrophin 2, 3 and 4.
The bestrophin family members are membrane protein with 2-TMD and have a conserved 350-400 amino domain including the invariant peptide motif RFP. Each of the Bestrophin proteins has a unique C-terminus that lack similarity to other proteins or motifs. Bestrophin 1 gene is localised to chromosome 19p13.2-p13.12, Bestrophin 2 to 1p32.3-p33 and Bestrophin 3 to 12q14.2-q15. RT-PCR analyses revealed tissue-restricted expression of the three genes with both Bestrophin 1 and Bestrophin 2 are abundantly transcribed in colon. Bestrophin 1 is present in the retinal pigment epithelium while Bestrophin 3 shows predominant expression in skeletal muscle (2, 3). Functionally the bestrophines oligomerise to form tetramers and pentamers in order to act as calcium sensitive chloride channels. It has been shown that Bestrophin interacts with beta catalytic subunit of protein phosphatase 2A (PP2Ac). Such Protein-protein interaction between bestrophin and PP2Ac and the structural subunit of PP2A, PR65, was confirmed by reciprocal immunoprecipitation. The interaction between PP2Ac and the Bestrophin takes place near the Carboxy-terminal end of the protein. Okadic acid induce the phosphorylation of Bestrophin in vitro. Bestrophin also serves in the signal transduction pathway that modulates the light peak of the EOG, that is regulated by phosphorylation of the Bestrophin that in turn is regulated by protein phosphatase 2A (PP2A).
Concentration 0.75 - 1.2 mg/ml (lot specific)
General Readings
  1. Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW, Marmorstein AD. J. Biol. Chemistry 2002; June 10, Electronic publication.
  2. Stohr H, Marquardt A, et. al., Eur J Hum Genet. 2002 Apr;10(4):281-4.
  3. Takashi Tsunenari , Hui Sun, John Williams, Hugh Cahill , Philip Smallwood, King-Wai Yau ** and Jeremy Nathans. J. Biol. Chem., Vol. 278, Issue 42, 41114-41125, October 17, 2003.
Storage Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
Stabilization buffer
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Mouse, rat
Specificity
Specificity:
This antibody detects Bestrophin-4 (C-term).
Gene ID 266675

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