AM26190PU-N Beta-2 adrenergic receptor antibody

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0.1 mg / €410.00
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Mouse anti Guinea Pig, Human, Rat Beta-2 adrenergic receptor 6H8

AM26190PU-N

Product Description for Beta-2 adrenergic receptor

Mouse anti Guinea Pig, Human, Rat Beta-2 adrenergic receptor 6H8.
Presentation: Purified
Product is tested for ELISA (detection), Enzyme Immunoassay, Functional assay.

Properties for Beta-2 adrenergic receptor

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms ADRB2, ADRB2R, B2AR, Beta-2 adrenoreceptor
Presentation Purified
Reactivity GP, Hu, Rt
Applications E, E(detection), FN
Clonality Monoclonal
Clone 6H8
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07550
Immunogen:
Free peptide Beta2-H19C
GeneID:
154
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Flow Cytometry: A431 Human epidermoid cells in suspension for 1 h at 4°C in PBS containing monoclonal Antibody 6H8 (260 nM). After 1 h of incubation, the cells were fixed with 2% Formaldehyde (Ref.1: Lebesgue). The typical starting working dilution is 1/50.
Functional Assays: Antibody 6H8 functions as an agonist in neonatal Rat cardiomyocytes (Ref 1, Lebesgue). Furthermore, Fab fragments of agonist-like antibody 6H8 behave as antagonist (Ref.2: Mijares).
Immunoassay: Useful as detector in an ELISA setting (Ref.1: Lebesgue).
Positive Control: Human epidermoid carcinoma cell A431 line.
Background

The b -adrenoceptors can be divided into b1, b2, b3 and b4-adrenoceptors defined in terms of agonist potencies, b2-adrenoceptors displayed a higher selectivity for nor-adrenaline than for adrenaline.
B2-receptors are mainly postsynaptic and are located on a number of tissues including blood vessels, bronchi, GIT, skeletal muscle, liver and mast cell. Activation results in vasodilatation, bronchodilation, relaxation of the GIT, glycogenolysis in the liver, tremor in skeletal muscle and inhibition of histamine release from mast cells.
Transduction is via G-proteins coupled to the intracellular second messenger adenylate cyclase. B-receptors are positively coupled to adenylate cyclase via activation of Gs G-protein, however activation of the b2-adrenoceptors results in stimulation and inhibition of adenylate cyclase. The b2-receptor selective agonists are widely used in the treatment of asthma and other related bronchospastic conditions. They are commonly used in the treatment of angina pectoris, cardiac arrhythmia and for the long-term treatment of patients who survive myocardial infarction. B-receptor antagonists have also been used as anti-hypertensive for a number of years. Beta -blockers have also proven useful in the treatment of conditions such as migraine, anxiety disorders, hyperthyroidism, alcohol withdrawal and when applied topically are useful in the treatment of glaucoma and ocular hypertension.

Concentration 0.1 mg/ml
General Readings
  1. Lebesgue D, Wallukat G, Mijares A, Granier C, Argibay J, Hoebeke J. An agonist-like monoclonal antibody against the human beta2-adrenoceptor. Eur J Pharmacol. 1998 May 1;348(1):123-33. PubMed PMID: 9650839.
  2. Mijares A, Lebesgue D, Wallukat G, Hoebeke J. From agonist to antagonist: Fab fragments of an agonist-like monoclonal anti-beta(2)-adrenoceptor antibody behave as antagonists. Mol Pharmacol. 2000 Aug;58(2):373-9. PubMed PMID: 10908305.
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS
Stabilizers:
0.1% BSA
State:
Liquid 0.2 µm filtered Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rat, Guinea Pig.
Specificity
Specificity:
The monoclonal antibody 6H8 recognizes Human beta-2-Adrenoceptor.
Gene ID 154
FocusOn and Reviews
FocusOns:

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Positive controls for Beta-2 adrenergic receptor (2 products)

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