R1064PS Beta-galactosidase tag antibody

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0.1 mg / €460.00
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Rabbit anti Escherichia coli Beta-galactosidase tag

R1064PS

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  • R1064PS

Product Description for Beta-galactosidase tag

Rabbit anti Escherichia coli Beta-galactosidase tag.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Beta-galactosidase tag

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag
Presentation Purified
Reactivity E. coli
Applications C, E, ICC/IF, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00722
Immunogen:
Full length native Beta-galactosidase isolated from E.coli
GeneID:
945006
Add. information Conjugates available. Please ask for details.
Application Suitable for Immunoblotting (Western or dot blot), ELISA, Immunofluorescence microscopy, Immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
Western blot: 1/5,000-1/10,000. A 1/5,000 dilution has been reported to be successful for staining by Immunoblot of Beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus). ELISA: 1/10,000.
Immunohistochemistry: 1/1,500. The antibody recognizes both Frozen tissue sections, Paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis:A 1/1,500 dilution has been reported to detect Beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections. A 1/5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1/10,000 dilution was noted to show no background and to be suitable for double labeling experiments). A 1/5,000 dilution has been reported to be successful for staining brain sections from transgenic mice expressing nuclear Beta-galactosidase when assayed by Immunofluorescence microscopy.
Background Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Originator or purchased from resellers:
  1. Hayworth CR, Moody SE, Chodosh LA, Krieg P, Rimer M, Thompson WJ. Induction of neuregulin signaling in mouse schwann cells in vivo mimics responses to denervation. J Neurosci. 2006 Jun 21;26(25):6873-84. PubMed PMID: 16793894.
General Readings
  1. Huber RE, Hakda S, Cheng C, Cupples CG, Edwards RA. Trp-999 of beta-galactosidase (Escherichia coli) is a key residue for binding, catalysis, and synthesis of allolactose, the natural lac operon inducer. Biochemistry. 2003 Feb 18;42(6):1796-803. PubMed PMID: 12578395.
  2. Matthews BW. The structure of E. coli beta-galactosidase. C R Biol. 2005 Jun;328(6):549-56. PubMed PMID: 15950161.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium azide
Stabilizers:
None
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This antibody detects Beta-galactosidase [E. coli]. Cross reactivity against Beta-galactosidase from other tissues and species may occur but have not been specifically determined. Very low background staining has been reported in various assays.
Immunoelectrophoresis gives a single precipitin arc against anti-rabbit serum as well as purified and partially purified Beta-galactosidase [E.coli].

Accessory Products

Proteins and/or Positive Controls

Proteins for Beta-galactosidase tag (3 products)

Catalog No. Species Pres. Purity   Source  

Beta-galactosidase tag

Beta-galactosidase tag E. coli 600 U/mg
5 mg / €390.00
  Acris Antibodies GmbH

Beta-galactosidase tag

Beta-galactosidase tag
  Acris Antibodies GmbH

Beta-galactosidase tag

Beta-galactosidase tag E. coli Purified
  Alpha Diagnostic Intl. Inc.
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