AM33308PU-N Biotin antibody

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0.2 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti All Species Biotin Hyb-8

Product Description for Biotin

Mouse anti All Species Biotin Hyb-8.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections, Flow Cytometry, Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Biotin

Product Category Primary Antibodies
Quantity 0.2 mg
Synonyms Vitamin B7, Vitamin H
Presentation Purified
Reactivity All Species
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone Hyb-8
Host Mouse
Isotype IgG1
Molecular weight 244kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Biotinylated Sheep immunoglobulin.
Isotype control AM03095PU-N
Application Western Blotting.
Confocal Microscopy. 
Flow Cytometry: 0.5-1 µg/106 cells. 
In situ Nucleic Acid Hybridization. 
Immunofluorescence: 0.5-1 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed 
Sections: 0.5-1 µg/ml for 30 minutes at RT. 
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Recommended Positive Control: Biotinylated proteins in solution or on tissues.
Concentration 0.2 mg/ml
General Readings
  1. Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, et al. Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1;181(9):5875-84. PubMed PMID: 18941176. (Free PMC Article available, 4 images available)
  2. Green NM, Moody KS, Debatis M, Marshak-Rothstein A. Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16;287(47):39789-99. doi: 10.1074/jbc.M112.383000. Epub 2012 Sep 27. PubMed PMID: 23019335. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin. 
This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise.
Cellular LocalizationDepends on the target.

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