AM33308PU-S Biotin antibody

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0.1 mg / €320.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti All Species Biotin Hyb-8

Product Description for Biotin

Mouse anti All Species Biotin Hyb-8.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections, Flow Cytometry, Frozen Sections, Immunocytochemistry/Immunofluorescence.

Properties for Biotin

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Vitamin B7, Vitamin H
Presentation Purified
Reactivity All Species
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone Hyb-8
Host Mouse
Isotype IgG1
Molecular weight 244kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Biotinylated Sheep immunoglobulin.
Isotype control AM03095PU-N
Application Western Blotting.
Confocal Microscopy. 
Flow Cytometry: 0.5-1 µg/106 cells. 
In situ Nucleic Acid Hybridization. 
Immunofluorescence: 0.5-1 µg/ml. 
Immunohistochemistry on Frozen and Formalin-Fixed 
Sections: 0.5-1 µg/ml for 30 minutes at RT. 
No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Recommended Positive Control: Biotinylated proteins in solution or on tissues.
Concentration 0.2 mg/ml
General Readings
  1. Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, et al. Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1;181(9):5875-84. PubMed PMID: 18941176. (Free PMC Article available, 4 images available)
  2. Green NM, Moody KS, Debatis M, Marshak-Rothstein A. Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16;287(47):39789-99. doi: 10.1074/jbc.M112.383000. Epub 2012 Sep 27. PubMed PMID: 23019335. (Free PMC Article available, 7 images available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of Biotin. 
This Monoclonal Antibody is highly specific to Biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for Biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-Biotin antibody may be substituted to decrease this noise.
Cellular LocalizationDepends on the target.

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