AP09429C2-N Biotin F(ab')2 antibody

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Goat anti Biotin F(ab')2


Product Description for Biotin F(ab')2

Goat anti Biotin F(ab')2.
Presentation: Cy2
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Biotin F(ab')2

Product Category Primary Antibodies
Quantity 1 mg
Presentation Cy2
Applications E, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Application Flow Cytometry: 1/200 - 1/400.
Immunofluorescence: 1/100 - 1/1,000.
Background Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Concentration 1,0 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
(Cyanine 2-OSu) (Molecular Weight 897 daltons)
Absorption emission:
489 nm / 505 nm
Molar radio:
2.1 moles Cy2 per mole of Goat IgG F(ab’)2
Immunoaffinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
Lyophilized F(ab)2 fragment
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage, add glycerol to 50%
This is a Cy2 conjugated F(ab)2 fragment of Anti-Biotin.

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